Erfan Randa, Shaker Olfat G, Khalil Mahmoud A F, Hassan Amel Raouf, Abu-El-Azayem Abeer K, Samy Amira, Abdelhamid Haitham, Awaji Aeshah A, El Sayed Hassan Salem, Mohammed Asmaa
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Fayoum University, Fayoum, 63514, Egypt.
Noncoding RNA Res. 2024 Aug 14;10:35-40. doi: 10.1016/j.ncrna.2024.08.005. eCollection 2025 Feb.
Alopecia areata (AA) commonly displays as non-scarring, irregular hair loss. Experimental and clinical research have specifically implicated autoimmunity and genetics in the disruption of anagen hair follicles. AA patients' scalp lesions and peripheral blood mononuclear cells (PBMCs) exhibited an immune state imbalance. Numerous studies attempt to establish a connection between the occurrence and prognosis of AA and the epigenetic modulation of gene expression by long noncoding RNA (lncRNA) and microRNA (miRNA). The current study aimed to examine the serum levels of nuclear enriched abundant transcript 1 (NEAT1) and its target miRNA101 (miR-101) in AA and investigate the ability to use them as diagnostic biomarkers in the disease.
Seventy-two AA patients were included in this prospective cohort study. Demographics, patient history, laboratory characteristics, and treatments were recorded. The miR-101 and NEAT1 levels were evaluated.
Serum NEAT1 levels were lower in AA patients, but there was no significant difference. However, there was no substantial disparity in NEAT1 level regarding other disease characteristics. There was a substantial positive association between NEAT1 and miR-101 levels among cases. On the other hand, the results showed a markedly low mean of miR-101 levels among patients, but the miR-101 marker shows no significant difference regarding different disease characteristics. The specificity and sensitivity test for the miR-101 marker shows a significant specificity of 60 % and sensitivity of 75 % with a p-value of 0.001 and a cut-off value of 0.897.
The current research determined that miR-101 works as a diagnostic biomarker for AA.
斑秃（AA）通常表现为非瘢痕性、不规则脱发。实验和临床研究明确表明自身免疫和遗传因素参与生长期毛囊的破坏。AA患者的头皮病变和外周血单个核细胞（PBMC）表现出免疫状态失衡。众多研究试图建立AA的发生、预后与长链非编码RNA（lncRNA）和微小RNA（miRNA）对基因表达的表观遗传调控之间的联系。本研究旨在检测AA患者血清中核富集丰富转录本1（NEAT1）及其靶标miRNA101（miR-101）的水平，并探讨将其用作该疾病诊断生物标志物的能力。
本前瞻性队列研究纳入了72例AA患者。记录人口统计学信息、患者病史、实验室特征和治疗情况。评估miR-101和NEAT1水平。
AA患者血清NEAT1水平较低，但无显著差异。然而，NEAT1水平在其他疾病特征方面无实质性差异。病例中NEAT1和miR-101水平之间存在显著正相关。另一方面，结果显示患者中miR-101水平的平均值明显较低，但miR-101标志物在不同疾病特征方面无显著差异。miR-101标志物的特异性和敏感性测试显示，特异性为60%，敏感性为75%，p值为0.001，截断值为0.897。
当前研究确定miR-101可作为AA的诊断生物标志物。
