Nanotechnology Engineering Department, Institute of Science, Bülent Ecevit University, Zonguldak, Turkey.
Vocational School of Eldivan Health Services, Karatekin University, Cankiri, Turkey.
J Biomater Appl. 2021 Sep;36(3):385-405. doi: 10.1177/0885328220983552. Epub 2021 Feb 2.
Nosocominal infections associated with biofilm formation on urinary catheters cause serious complications. The aim of this study was to investigate the feasibility of the polyurethane (PU) catheter modified with tetracycline hydrochloride (TCH) attached Ag nanoparticles embedded PolyRicinoleic acid-Polystyrene Nanoparticles (PU-TCH-AgNPs-PRici-PS NPs) and the influence on antimicrobial and antibiofilm activity of urinary catheters infected by and . For this purpose, AgNPs embedded PRici graft PS graft copolymers (AgNPs-PRici-g-PS) were synthesized via free radical polymerization and characterized by FTIR, HNMR and DSC. AgNPs-PRici-PS NPs were prepared and optimized by the different parameters and the optimized size of nanoparticle was found as about 150 ± 1 nm. The characterization of the nanoparticles and the morphological evaluation were carried out by FTIR and SEM. Short term stability of nanoparticles was realised at 4°C for 30 days. release profiles of TCH and Ag NPs were also investigated. The formation of biofilm on PU modified TCH-Ag NPs-PRici-PS NPs, was evaluated and the biocompatibility test of the nanoparticles was realized via the mouse fibroblast (L929) and mouse urinary bladder cells (G/G An1). This is the first time that TCH-AgNPs-PRici-PS NPs used in the modification of PU catheter demonstrated high antimicrobial and antibiofilm activities against the urinary tract infection.
与生物膜形成相关的医院感染会导致严重的并发症。本研究旨在探讨载盐酸四环素（TCH）的银纳米粒子嵌入聚蓖麻酸-聚苯乙烯纳米粒子（PU-TCH-AgNPs-PRici-PS NPs）改性的聚氨酯（PU）导管的可行性及其对 和 感染的导尿管的抗菌和抗生物膜活性的影响。为此，通过自由基聚合合成了嵌入 AgNPs 的 PRici 接枝 PS 接枝共聚物（AgNPs-PRici-g-PS），并通过 FTIR、HNMR 和 DSC 进行了表征。通过不同的参数制备和优化了 AgNPs-PRici-PS NPs，优化后的纳米颗粒尺寸约为 150±1nm。通过 FTIR 和 SEM 对纳米颗粒进行了表征和形态学评价。在 4°C 下 30 天内实现了纳米颗粒的短期稳定性。还研究了 TCH 和 Ag NPs 的 释放曲线。评估了在 PU 改性的 TCH-Ag NPs-PRici-PS NPs 上形成的生物膜，并通过小鼠成纤维细胞（L929）和小鼠膀胱细胞（G/G An1）进行了纳米颗粒的生物相容性试验。这是首次将 TCH-AgNPs-PRici-PS NPs 用于改性 PU 导管，表现出对尿路感染的高抗菌和抗生物膜活性。
