Puertas-Segura Antonio, Morena Angela Gala, Pérez Rafael Silvia, Ivanova Kristina, Ivanov Ivan, Todorova Katerina, Dimitrov Petar, Ciardelli Gianluca, Tzanov Tzanko
Grup de Biotecnologia Molecular i Industrial, Department of Chemical Engineering, Universitat Politècnica de Catalunya, Rambla Sant Nebridi 22, 08222 Terrassa, Spain.
Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Geo Milev, 1113 Sofia, Bulgaria.
ACS Appl Mater Interfaces. 2024 Jul 31;16(30):39129-39139. doi: 10.1021/acsami.4c08599. Epub 2024 Jul 23.
Catheter-associated urinary tract infections represent a major share of nosocomial infections, and are associated with longer periods of hospitalization and a huge financial burden. Currently, there are only a handful of commercial materials that reduce biofilm formation on urinary catheters, mostly relying on silver alloys. Therefore, we combined silver-phenolated lignin nanoparticles with poly(carboxybetaine) zwitterions to build a composite antibiotic-free coating with bactericidal and antifouling properties. Importantly, the versatile lignin chemistry enabled the formation of the coating in situ, enabling both the nanoparticle grafting and the radical polymerization by using only the oxidative activity of laccase. The resulting surface efficiently prevented nonspecific protein adsorption and reduced the bacterial viability on the catheter surface by more than 2 logs under hydrodynamic flow, without exhibiting any apparent signs of cytotoxicity. Moreover, the said functionality was maintained over a week both in vitro and in vivo, whereby the animal models showed excellent biocompatibility.
导尿管相关的尿路感染占医院感染的很大比例，并且与更长的住院时间和巨大的经济负担相关。目前，仅有少数几种商业材料可减少导尿管上生物膜的形成，主要依赖于银合金。因此，我们将银酚化木质素纳米颗粒与聚（羧酸甜菜碱）两性离子相结合，构建了一种具有杀菌和防污性能的无抗生素复合涂层。重要的是，通用的木质素化学性质使得能够原位形成涂层，仅利用漆酶的氧化活性就可实现纳米颗粒接枝和自由基聚合。所得表面有效地防止了非特异性蛋白质吸附，并在流体动力流下使导管表面的细菌活力降低了超过2个对数，且未表现出任何明显的细胞毒性迹象。此外，所述功能在体外和体内均可维持一周以上，动物模型显示出优异的生物相容性。
