Yu Ping, Li Qian, Feng Yanmei, Chen Yuying, Ma Sinan, Ding Xiaoqin
Gansu Provincial Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
J Anal Methods Chem. 2021 Jan 18;2021:6655572. doi: 10.1155/2021/6655572. eCollection 2021.
To establish a method for simultaneous determination of liquiritin, liquiritigenin, and isoliquiritinin glycyrrhizin using hydrogen nuclear magnetic resonance quantitative technology (H-qNMR). . Deuterated dimethyl sulfoxide was used as the solvent, and dichloromethane was used as the internal standard. The probe temperature was 298.0 K, the pulse sequence was Zg30, the number of scans was 16, and relaxation delay (1) was 10 s. Quantitative characteristic signal peaks were 4.891∼4.878 ppm, 8.187∼8.172 ppm, and 6.790∼6.776 ppm for liquiritin, isoliquiritin, and liquiritigenin, respectively.
The experimental result showed that the content of flavonoids in Licorice, from Chifeng, Inner Mongolia, was the highest.
In this study, a new method for determination of three flavonoids in Licorice using H-qNMR was established. This experimental method has the advantages of accuracy, efficiency, and economy. It lays a foundation for the study on the determination of flavonoids content in licorice by proton nuclear magnetic resonance spectroscopy.
建立一种利用氢核磁共振定量技术（H-qNMR）同时测定甘草中甘草苷、甘草素和异甘草苷以及甘草酸的方法。以氘代二甲基亚砜为溶剂，二氯甲烷为内标。探头温度为298.0 K，脉冲序列为Zg30，扫描次数为16，弛豫延迟（1）为10 s。甘草苷、异甘草苷和甘草素的定量特征信号峰分别为4.891∼4.878 ppm、8.187∼8.172 ppm和6.790∼6.776 ppm。
实验结果表明，内蒙古赤峰产甘草中黄酮类化合物含量最高。
本研究建立了一种利用H-qNMR测定甘草中三种黄酮类化合物的新方法。该实验方法具有准确、高效、经济的优点。为质子核磁共振光谱法测定甘草中黄酮类化合物含量的研究奠定了基础。
