Drug Discovery and Development Centre, Office of Advanced Science and Technology, Thammasat University (Rangsit Campus), Pathumthani, Thailand.
Department of Chemistry, Faculty of Science and Technology, Thammasat University (Rangsit Campus), Pathumthani, Thailand.
Phytochem Anal. 2019 Nov;30(6):617-622. doi: 10.1002/pca.2834. Epub 2019 Apr 24.
Quantitative nuclear magnetic resonance (qNMR) spectroscopy is an analytical method based on the principles of NMR spectroscopy. The main advantages of this method are its simplicity, time efficiency, high accuracy and reproducibility, and it is a non-destructive technique.
To evaluate and standardise the quality of Artocarpus lacucha heartwood. A method for quantifying its oxyresveratrol content using qNMR was developed.
Proton ( H)NMR (400 MHz) spectroscopy was used to analyse the methanol-d solution of a given amount of crude extract of A. lacucha heartwood using ethyl p-methoxycinnamate (EPMC) as an internal standard. The qNMR methodology was validated in terms of its linearity and range, limit of quantification (LOQ), stability, precision, and accuracy for the determination of the oxyresveratrol content.
The qNMR method was validated in terms of its linearity, range, LOQ, accuracy, precision, and stability. The quantitative determination of the oxyresveratrol content in the methanolic crude extract of A. lacucha was found to be 17% based on HNMR analysis, which proved to be a reliable method as the results were comparable to those obtained by high-performance liquid chromatography (HPLC) analysis.
This study validated qNMR spectroscopy as a reliable analytical procedure to determine oxyresveratrol in A. lacucha heartwood. Therefore, this qNMR method can serve as an alternative to the classical HPLC methods for evaluating and standardising the quality of A. lacucha heartwood.
定量核磁共振（qNMR）光谱学是一种基于核磁共振光谱学原理的分析方法。该方法的主要优点是简单、省时、准确度和重现性高，且为非破坏性技术。
评估和标准化暗罗心材的质量。开发了一种使用 qNMR 定量测定其白藜芦醇含量的方法。
采用质子（ H）NMR（400 MHz）光谱法，使用乙基对甲氧基肉桂酸酯（EPMC）作为内标，分析一定量暗罗心材甲醇粗提物的甲醇-d 溶液。从线性和范围、定量限（LOQ）、稳定性、精密度和准确度等方面对 qNMR 方法进行验证，以确定白藜芦醇含量。
qNMR 方法在其线性、范围、LOQ、准确度、精密度和稳定性方面得到了验证。基于 1 HNMR 分析，暗罗甲醇粗提物中白藜芦醇的定量测定含量为 17%，这证明是一种可靠的方法，因为结果与高效液相色谱（HPLC）分析的结果相当。
本研究验证了 qNMR 光谱学作为一种可靠的分析方法，可用于测定暗罗心材中的白藜芦醇。因此，该 qNMR 方法可以替代经典的 HPLC 方法，用于评估和标准化暗罗心材的质量。
