利用原子力显微镜测量微环境调控的癌细胞核硬度。

Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India.

Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100, Israel.

STAR Protoc. 2021 Jan 22;2(1):100296. doi: 10.1016/j.xpro.2021.100296. eCollection 2021 Mar 19.

Quantification of nuclear stiffness is challenging for cells encapsulated within a 3D extracellular matrix (ECM). Here, we describe an experimental setup for measuring microenvironment-dependent tuning of nuclear stiffness using an atomic force microscope (AFM). In our setup, ECM-coated polyacrylamide hydrogels mimic the stiffness of the microenvironment, enabling the measurement of nuclear stiffness using an AFM probe in live cancer cells. For complete details on the use and execution of this protocol, please refer to Das et al. (2019) (https://doi.org/10.1016/j.matbio.2019.01.001).

量化被 3D 细胞外基质（ECM）包裹的细胞的核硬度极具挑战性。在此，我们描述了一种使用原子力显微镜（AFM）测量微环境依赖性核硬度调节的实验设置。在我们的设置中，ECM 包被的聚丙烯酰胺水凝胶模拟微环境的硬度，使我们能够使用 AFM 探针在活癌细胞中测量核硬度。有关该方案使用和执行的完整详细信息，请参阅 Das 等人（2019 年）（https://doi.org/10.1016/j.matbio.2019.01.001）。