Acta Chim Slov. 2020 Dec;67(4):1250-1261.
The effect of homogenization, ultrasound and microwave extraction methods and conditions on fucoxanthin content, total phenolic content and antioxidant activity of extracts obtained from Phaeodactylum tricornutum were investigated in this study. The solvent/biomass ratio was the most effective parameter on fucoxanthin content, total phenolic content and antioxidant activity. The maximum fucoxanthin content (5.60 ± 0.06 mg/g) and antioxidant activity (763.00 ± 15.88 EC50 ?g/mL extract) were obtained with the homogenization extraction method whose optimum conditions were 1.93% biomass/solvent ratio, ~5200 rpm homogenization rate and 14.2 min extraction time. Although the ultrasonic extraction method has reached the approximately same level of fucoxanthin content (5.24 ± 0.07 mg/g)), TPC (67.68 ± 1.58 mg gallic acid/L) and antioxidant activity (619.90 ± 17.16 EC50 ?g/mL extract) at an amplitude of 55.72%, a higher biomass/ solvent ratio (2.72%) and a longer extraction time (17.37 min) have been required. The lowest fucoxanthin content, total phenolic content and antioxidant activity were determined for the microwave extraction method.
本研究考察了匀浆、超声和微波提取方法及条件对三角褐指藻中岩藻黄质含量、总酚含量和提取物抗氧化活性的影响。溶剂/生物量比是影响岩藻黄质含量、总酚含量和抗氧化活性的最有效参数。采用匀浆提取法，在生物量/溶剂比为 1.93%、匀浆速率约为 5200 rpm、提取时间为 14.2 min 的最佳条件下，可获得最大的岩藻黄质含量（5.60 ± 0.06 mg/g）和抗氧化活性（763.00 ± 15.88 EC50?g/mL 提取物）。虽然超声提取法的岩藻黄质含量（5.24 ± 0.07 mg/g）、总酚含量（67.68 ± 1.58 mg 没食子酸/L）和抗氧化活性（619.90 ± 17.16 EC50?g/mL 提取物）达到了相近的水平，但需要更高的生物量/溶剂比（2.72%）和更长的提取时间（17.37 min）。微波提取法得到的岩藻黄质含量、总酚含量和抗氧化活性最低。
