Beijing Research Center for Agricultural Standards and Testing, No. 9 Middle Road of Shuguanghuayuan, Haidian Dist., Beijing, 100097, China.
Analyst. 2021 Mar 21;146(6):1793-1806. doi: 10.1039/d0an02171g. Epub 2021 Feb 3.
Mycotoxins are toxic secondary metabolites naturally produced by fungi. They can cause various kinds of acute and chronic diseases in both humans and animals since food usually contains trace amounts of mycotoxins. Thus, it is important to develop a rapid and sensitive technique for mycotoxin detection. Except for the original and classical enzyme-linked immunosorbent assays (ELISA), a series of biosensors has been developed to analyze mycotoxins in food in the last decade with the advantages of rapid analysis, simplicity, portability, reproducibility, stability, accuracy, and low cost. Nanomaterials have been incorporated into biosensors for the purpose of achieving better analytical performance in terms of limit of detection, linear range, analytical stability, low production cost, etc. Gold nanoparticles (AuNPs) are one of the most extensively studied and commonly used nanomaterials, which can be employed as an immobilization carrier, signal amplifier, mediator and mimic enzyme label. This paper aims to present an extensive overview of the recent progress in AuNPs in mycotoxin detection through ELISA and biosensors. The details of the detection methods and their application principles are described, and current challenges and future prospects are discussed as well.
真菌自然产生的有毒次级代谢物即为霉菌毒素。由于食物中通常含有微量的霉菌毒素，因此它们会导致人类和动物患上各种急性和慢性疾病。因此，开发一种快速灵敏的霉菌毒素检测技术非常重要。除了最初的经典酶联免疫吸附测定（ELISA）之外，在过去十年中，已经开发出一系列生物传感器来分析食品中的霉菌毒素，这些生物传感器具有快速分析、简单、便携、可重复性、稳定性、准确性和低成本等优点。为了在检测限、线性范围、分析稳定性、低成本等方面获得更好的分析性能，生物传感器中已经加入了纳米材料。金纳米粒子（AuNPs）是研究和应用最广泛的纳米材料之一，可作为固定化载体、信号放大剂、介体和模拟酶标记。本文旨在通过 ELISA 和生物传感器对金纳米粒子在霉菌毒素检测中的最新进展进行广泛综述。文中详细描述了检测方法及其应用原理，并讨论了当前的挑战和未来的前景。
