Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Biosens Bioelectron. 2019 Oct 15;143:111603. doi: 10.1016/j.bios.2019.111603. Epub 2019 Aug 19.
Mycotoxins are highly toxic secondary metabolites, which may contaminate many types of food and feeds. These toxins have serious health risks for both human and animals. One of the effective ways to prevent food contamination and protect people against mycotoxins is based on timely detection. Several methods like enzyme-linked immunosorbent assay and affinity chromatography are commercially available for this purpose. Nevertheless, sensitive, fast, simple, low-cost, and portable devices are absolutely required for a fast point-of care information and making decisions. Application of biosensors appears to be a possible technique to meet this need for mycotoxins analyze. The present study has been focused on the literature update of smart using of biosensing for detection of mycotoxin at both academic and industrial levels in order to replace conventional chromatographic methods. Surface plasmon resonance (SPR) as one of the relatively novel and simple analytical method has been proven for rapid, sensitive, label-free detection and widely used for qualitative and quantitative analysis of multiplexed pollutant in real-time. This paper aims to provide an extensive overview on biosensors for mycotoxin detection by highlighting the main biorecognition elements. Moreover, SPR principles, assay formats, and signal enhancement are summarized.
真菌毒素是高度有毒的次级代谢物，可能污染许多类型的食物和饲料。这些毒素对人类和动物的健康都有严重的风险。预防食物污染和保护人们免受真菌毒素侵害的有效方法之一是基于及时检测。酶联免疫吸附测定和亲和层析等几种方法可用于此目的。然而，对于快速的即时信息和决策，需要灵敏、快速、简单、低成本和便携式的设备。生物传感器的应用似乎是满足这一需求的一种可行技术，用于分析真菌毒素。本研究重点关注智能使用生物传感器在学术和工业水平上检测真菌毒素的文献更新，以取代传统的色谱方法。表面等离子体共振（SPR）作为一种相对新颖和简单的分析方法，已被证明可用于快速、灵敏、无标记检测，并广泛用于实时定性和定量分析多种污染物。本文旨在通过突出主要的生物识别元件，提供生物传感器检测真菌毒素的广泛概述。此外，还总结了 SPR 的原理、分析形式和信号增强。
