Department of Orthodontics, University Medical Centre of Regensburg, D-93053 Regensburg, Germany.
Centre for Medical Biotechnology, Department of Orthopaedic Surgery, Experimental Orthopaedics, University of Regensburg, D-93053 Regensburg, Germany.
Cells. 2021 Feb 1;10(2):298. doi: 10.3390/cells10020298.
Genetic predisposition, traumatic events, or excessive mechanical exposure provoke arthritic changes in the temporomandibular joint (TMJ). We analysed the impact of mechanical stress that might be involved in the development and progression of TMJ osteoarthritis (OA) on murine synovial fibroblasts (SFs) of temporomandibular origin. SFs were subjected to different protocols of mechanical stress, either to a high-frequency tensile strain for 4 h or to a tensile strain of varying magnitude for 48 h. The TMJ OA induction was evaluated based on the gene and protein secretion of inflammatory factors (, , , , , , , PG-E2), subchondral bone remodelling (, ), and extracellular matrix components (, , collagen and hyaluronic acid deposition) using RT-qPCR, ELISA, and HPLC. A short high-frequency tensile strain had only minor effects on inflammatory factors and no effects on the subchondral bone remodelling induction or matrix constituent production. A prolonged tensile strain of moderate and advanced magnitude increased the expression of inflammatory factors. An advanced tensile strain enhanced the and PG-E2 expression, while the expression of further inflammatory factors were decreased. The tensile strain protocols had no effects on the expression, while the advanced tensile strain significantly reduced the deposition of matrix constituent contents of collagen and hyaluronic acid. The data indicates that the application of prolonged advanced mechanical stress on SFs promote PG-E2 protein secretion, while the deposition of extracellular matrix components is decreased.
遗传易感性、创伤事件或过度的机械暴露会引发颞下颌关节（TMJ）的关节炎变化。我们分析了可能参与 TMJ 骨关节炎（OA）发展和进展的机械应力对颞下颌来源的滑膜成纤维细胞（SF）的影响。SF 经历了不同的机械应力方案，要么是 4 小时的高频拉伸应变，要么是 48 小时的不同幅度的拉伸应变。基于炎症因子（、、、、、、PG-E2）、软骨下骨重塑（、）和细胞外基质成分（、、胶原和透明质酸沉积）的基因和蛋白分泌，评估 TMJ OA 诱导，使用 RT-qPCR、ELISA 和 HPLC。短暂的高频拉伸应变仅对炎症因子有较小的影响，对软骨下骨重塑诱导或基质成分产生没有影响。适度和高级幅度的延长拉伸应变增加了炎症因子的表达。高级拉伸应变增强了和 PG-E2 的表达，而进一步的炎症因子的表达减少。拉伸应变方案对的表达没有影响，而高级拉伸应变显著降低了胶原和透明质酸的细胞外基质成分的沉积。数据表明，SF 上延长的高级机械应力的应用促进了 PG-E2 蛋白的分泌，同时降低了细胞外基质成分的沉积。
