Al Mahmud Hafij, Baishya Jiwasmika, Wakeman Catherine A
Department of Biological Sciences, Texas Tech University, Lubbock, TX 2901, USA.
Pathogens. 2021 Feb 1;10(2):146. doi: 10.3390/pathogens10020146.
Cystic fibrosis (CF) is a genetic disease frequently associated with chronic lung infections caused by a consortium of pathogens. It is common for auxotrophy (the inability to biosynthesize certain essential metabolites) to develop in clinical isolates of the dominant CF pathogen , indicating that the CF lung environment is replete in various nutrients. Many of these nutrients are likely to come from the host tissues, but some may come from the surrounding polymicrobial community within the lungs of CF patients as well. To assess the feasibility of nutrient exchange within the polymicrobial community of the CF lung, we selected and , two of the most prevalent species found in the CF lung environment. By comparing the polymicrobial culture of wild-type strains relative to their purine auxotrophic counterparts, we were able to observe metabolic complementation occurring in both and when grown with a purine-producing cross-species pair. While our data indicate that some of this complementation is likely derived from extracellular DNA freed by lysis of by the highly competitive , the partial complementation of purine deficiency by demonstrates that bidirectional nutrient exchange between these classic competitors is possible.
囊性纤维化（CF）是一种遗传性疾病，常与由多种病原体引起的慢性肺部感染相关。在主要的CF病原体的临床分离株中，营养缺陷型（无法生物合成某些必需代谢物）很常见，这表明CF肺部环境富含各种营养物质。这些营养物质中的许多可能来自宿主组织，但也有一些可能来自CF患者肺部周围的多微生物群落。为了评估CF肺部多微生物群落中营养物质交换的可行性，我们选择了 和 ，它们是在CF肺部环境中发现的两种最常见的物种。通过比较野生型菌株与其嘌呤营养缺陷型对应物的多微生物培养物，我们能够观察到当与产生嘌呤的跨物种对一起生长时， 和 中都发生了代谢互补。虽然我们的数据表明这种互补作用部分可能源自被高度竞争性的 裂解后释放的细胞外DNA，但 对 嘌呤缺乏的部分互补表明这些典型竞争者之间双向营养物质交换是可能的。
