• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

甲基化改变基因的鉴定及其在脓毒症诱导的急性呼吸窘迫综合征早期诊断中的作用

Identification of Genes with Altered Methylation and Its Role in Early Diagnosis of Sepsis-Induced Acute Respiratory Distress Syndrome.

作者信息

Feng Jihua, Pang Jielong, He Dan, Wu Zimeng, Li Qian, Ji Pan, He Cuiying, Zhong Zhimei, Li Hongyuan, Zhang Jianfeng

机构信息

Department of Emergency Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, People's Republic of China.

出版信息

Int J Gen Med. 2021 Jan 25;14:243-253. doi: 10.2147/IJGM.S287960. eCollection 2021.

DOI:10.2147/IJGM.S287960
PMID:33536775
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7847772/
Abstract

PURPOSE

Early diagnosis of sepsis-induced acute respiratory distress syndrome (ARDS) is critical for effective treatment. We aimed to identify early stage biomarkers.

MATERIALS AND METHODS

Differentially expressed genes were identified in whole blood samples from patients with sepsis or ARDS based on the Gene Expression Omnibus (GEO) datasets GSE32707, GSE54514 and GSE10361. Functional enrichment analysis explored the biological characteristics of differentially expressed genes. Genes with high functional connectivity based on a protein-protein interaction network were marked as hub genes, which were validated using the GEO dataset GSE76293, and a gene set variation analysis index (GSVA) was assigned. Diagnostic and predictive ability of the hub genes were assessed by receiver operating characteristic (ROC) curve analysis. DNA methylation levels of hub genes were quantified using the GEO dataset GSE67530.

RESULTS

Forty-one differentially expressed genes were shared between sepsis-specific and ARDS-specific datasets. MAP2K2 and IRF7 functional activity was highly connected in sepsis-induced ARDS. Hub genes included RETN, MVP, DEFA4, CTSG, AZU1, FMNL1, RBBP7, POLD4, RIN3, IRF7. ROC curve analysis of the hub gene GSVA index showed good diagnostic ability in sepsis or ARDS. Among genes related to sepsis-induced ARDS, 17 were differentially methylated. Principal component analysis and heatmaps indicated that gene methylation patterns differed significantly between ARDS patients and controls.

CONCLUSION

We identified a genetic profile specific to early-stage sepsis-induced ARDS. The abnormal expression of these genes may be caused by hypomethylation, which may serve as a biomarker for early diagnosis of ARDS.

摘要

目的

脓毒症诱导的急性呼吸窘迫综合征（ARDS）的早期诊断对于有效治疗至关重要。我们旨在识别早期生物标志物。

材料与方法

基于基因表达综合数据库（GEO）数据集GSE32707、GSE54514和GSE10361，在脓毒症或ARDS患者的全血样本中识别差异表达基因。功能富集分析探索差异表达基因的生物学特征。基于蛋白质-蛋白质相互作用网络具有高功能连通性的基因被标记为枢纽基因，使用GEO数据集GSE76293对其进行验证，并赋予基因集变异分析指数（GSVA）。通过受试者工作特征（ROC）曲线分析评估枢纽基因的诊断和预测能力。使用GEO数据集GSE67530对枢纽基因的DNA甲基化水平进行定量。

结果

脓毒症特异性和ARDS特异性数据集之间共有41个差异表达基因。MAP2K2和IRF7的功能活性在脓毒症诱导的ARDS中高度连通。枢纽基因包括RETN、MVP、DEFA4、CTSG、AZU1、FMNL1、RBBP7、POLD4、RIN3、IRF7。枢纽基因GSVA指数的ROC曲线分析显示在脓毒症或ARDS中具有良好的诊断能力。在与脓毒症诱导的ARDS相关的基因中，17个基因存在差异甲基化。主成分分析和热图表明ARDS患者和对照组之间的基因甲基化模式存在显著差异。

结论

我们识别出了早期脓毒症诱导的ARDS特有的基因谱。这些基因的异常表达可能由低甲基化引起，这可能作为ARDS早期诊断的生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/a678562ea975/IJGM-14-243-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/bf6b17f092c6/IJGM-14-243-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/d3bab23616b0/IJGM-14-243-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/97dd10e27825/IJGM-14-243-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/2eb96fa10f83/IJGM-14-243-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/be3a9309a426/IJGM-14-243-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/a678562ea975/IJGM-14-243-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/bf6b17f092c6/IJGM-14-243-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/d3bab23616b0/IJGM-14-243-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/97dd10e27825/IJGM-14-243-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/2eb96fa10f83/IJGM-14-243-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/be3a9309a426/IJGM-14-243-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6be/7847772/a678562ea975/IJGM-14-243-g0006.jpg

相似文献

1
Identification of Genes with Altered Methylation and Its Role in Early Diagnosis of Sepsis-Induced Acute Respiratory Distress Syndrome.甲基化改变基因的鉴定及其在脓毒症诱导的急性呼吸窘迫综合征早期诊断中的作用
Int J Gen Med. 2021 Jan 25;14:243-253. doi: 10.2147/IJGM.S287960. eCollection 2021.
2
Identification and Validation of Autophagy-Related Genes in Sepsis-Induced Acute Respiratory Distress Syndrome and Immune Infiltration.脓毒症诱导的急性呼吸窘迫综合征中自噬相关基因的鉴定与验证及免疫浸润
J Inflamm Res. 2022 Apr 5;15:2199-2212. doi: 10.2147/JIR.S355225. eCollection 2022.
3
Identification of an Alveolar Macrophage-Related Core Gene Set in Acute Respiratory Distress Syndrome.急性呼吸窘迫综合征中肺泡巨噬细胞相关核心基因集的鉴定
J Inflamm Res. 2021 Jun 1;14:2353-2361. doi: 10.2147/JIR.S306136. eCollection 2021.
4
A hub gene signature as a therapeutic target and biomarker for sepsis and geriatric sepsis-induced ARDS concomitant with COVID-19 infection.一种作为治疗靶点和生物标志物的枢纽基因特征，用于脓毒症和老年脓毒症合并 COVID-19 感染引起的 ARDS。
Front Immunol. 2023 Sep 26;14:1257834. doi: 10.3389/fimmu.2023.1257834. eCollection 2023.
5
Identification of potential biomarkers and therapeutic targets for posttraumatic acute respiratory distress syndrome.创伤后急性呼吸窘迫综合征潜在生物标志物和治疗靶点的鉴定。
BMC Med Genomics. 2023 Mar 14;16(1):54. doi: 10.1186/s12920-023-01482-2.
6
Integrated Analysis of Gene Co-Expression Network and Prediction Model Indicates Immune-Related Roles of the Identified Biomarkers in Sepsis and Sepsis-Induced Acute Respiratory Distress Syndrome.基于基因共表达网络的综合分析和预测模型表明，鉴定的生物标志物在脓毒症和脓毒症诱导的急性呼吸窘迫综合征中的免疫相关作用。
Front Immunol. 2022 Jun 30;13:897390. doi: 10.3389/fimmu.2022.897390. eCollection 2022.
7
Consensus analysis via weighted gene co-expression network analysis (WGCNA) reveals genes participating in early phase of acute respiratory distress syndrome (ARDS) induced by sepsis.通过加权基因共表达网络分析（WGCNA）进行共识分析，揭示了参与脓毒症诱导的急性呼吸窘迫综合征（ARDS）早期阶段的基因。
Bioengineered. 2021 Dec;12(1):1161-1172. doi: 10.1080/21655979.2021.1909961.
8
DNA methylation exploration for ARDS: a multi-omics and multi-microarray interrelated analysis.用于 ARDS 的 DNA 甲基化探索：一种多组学和多微阵列相互关联的分析。
J Transl Med. 2019 Oct 17;17(1):345. doi: 10.1186/s12967-019-2090-1.
9
Bioinformatics and system biology approach to identify the influences among COVID-19, ARDS and sepsis.生物信息学和系统生物学方法识别 COVID-19、ARDS 和脓毒症之间的影响。
Front Immunol. 2023 May 16;14:1152186. doi: 10.3389/fimmu.2023.1152186. eCollection 2023.
10
Identification and validation of autophagy-related genes in exogenous sepsis-induced acute respiratory distress syndrome.鉴定和验证外源性脓毒症诱导的急性呼吸窘迫综合征中的自噬相关基因。
Immun Inflamm Dis. 2022 Oct;10(10):e691. doi: 10.1002/iid3.691.

引用本文的文献

1
Epigenetic Regulation of Neutrophils in ARDS.急性呼吸窘迫综合征中中性粒细胞的表观遗传调控
Cells. 2025 Jul 25;14(15):1151. doi: 10.3390/cells14151151.
2
Exploring ENPP5 as a diagnostic biomarker for sepsis: a comprehensive bioinformatics analysis.探索ENPP5作为脓毒症的诊断生物标志物：一项全面的生物信息学分析
BMC Infect Dis. 2025 Jul 1;25(1):831. doi: 10.1186/s12879-025-11152-6.
3
Methods in DNA methylation array dataset analysis: A review.DNA甲基化阵列数据集分析方法：综述

本文引用的文献

1
IRF3 and IRF7 contribute to diesel exhaust particles-induced pulmonary inflammation by mediating mTORC1 activation and restraining autophagy in mice.IRF3 和 IRF7 通过介导 mTORC1 的激活和抑制自噬来促进柴油机排气颗粒诱导的肺部炎症。
Eur J Immunol. 2020 Aug;50(8):1142-1153. doi: 10.1002/eji.201948415. Epub 2020 Mar 29.
2
Global transcriptional regulation of STAT3- and MYC-mediated sepsis-induced ARDS.STAT3 和 MYC 介导的脓毒症诱导的 ARDS 的全局转录调控。
Ther Adv Respir Dis. 2019 Jan-Dec;13:1753466619879840. doi: 10.1177/1753466619879840.
3
Analysis of transcription factor- and ncRNA-mediated potential pathogenic gene modules in Alzheimer's disease.
Comput Struct Biotechnol J. 2024 May 17;23:2304-2325. doi: 10.1016/j.csbj.2024.05.015. eCollection 2024 Dec.
4
The Role of RIN3 Gene in Alzheimer's Disease Pathogenesis: a Comprehensive Review.RIN3 基因在阿尔茨海默病发病机制中的作用：全面综述。
Mol Neurobiol. 2024 Jun;61(6):3528-3544. doi: 10.1007/s12035-023-03802-0. Epub 2023 Nov 23.
5
Single-Cell RNA Sequencing and Transcriptome Analysis Revealed the Immune Microenvironment and Gene Markers of Acute Respiratory Distress Syndrome.单细胞RNA测序与转录组分析揭示急性呼吸窘迫综合征的免疫微环境及基因标志物
J Inflamm Res. 2023 Aug 1;16:3205-3217. doi: 10.2147/JIR.S419576. eCollection 2023.
6
Genetic Determinants of the Acute Respiratory Distress Syndrome.急性呼吸窘迫综合征的遗传决定因素
J Clin Med. 2023 May 27;12(11):3713. doi: 10.3390/jcm12113713.
7
Single-cell analysis reveals dysregulated inflammatory response in peripheral blood immunity in patients with acute respiratory distress syndrome.单细胞分析揭示急性呼吸窘迫综合征患者外周血免疫中炎症反应失调。
Front Cell Dev Biol. 2023 May 22;11:1199122. doi: 10.3389/fcell.2023.1199122. eCollection 2023.
8
Acute Respiratory Distress Syndrome; A Review of Recent Updates and a Glance into the Future.急性呼吸窘迫综合征：近期进展回顾与未来展望
Diagnostics (Basel). 2023 Apr 24;13(9):1528. doi: 10.3390/diagnostics13091528.
9
The Programmed Cell Death of Macrophages, Endothelial Cells, and Tubular Epithelial Cells in Sepsis-AKI.脓毒症相关性急性肾损伤中巨噬细胞、内皮细胞和肾小管上皮细胞的程序性细胞死亡
Front Med (Lausanne). 2021 Dec 2;8:796724. doi: 10.3389/fmed.2021.796724. eCollection 2021.
10
Identification of an Alveolar Macrophage-Related Core Gene Set in Acute Respiratory Distress Syndrome.急性呼吸窘迫综合征中肺泡巨噬细胞相关核心基因集的鉴定
J Inflamm Res. 2021 Jun 1;14:2353-2361. doi: 10.2147/JIR.S306136. eCollection 2021.
阿尔茨海默病中转录因子和非编码RNA介导的潜在致病基因模块分析
Aging (Albany NY). 2019 Aug 16;11(16):6109-6119. doi: 10.18632/aging.102169.
4
Histone Methylation Mechanisms Modulate the Inflammatory Response of Periodontal Ligament Progenitors.组蛋白甲基化机制调节牙周膜祖细胞的炎症反应。
Stem Cells Dev. 2019 Aug 1;28(15):1015-1025. doi: 10.1089/scd.2019.0125. Epub 2019 Jul 22.
5
ATF4 Deficiency Promotes Intestinal Inflammation in Mice by Reducing Uptake of Glutamine and Expression of Antimicrobial Peptides.转录激活因子 4 缺乏通过减少谷氨酰胺摄取和抗菌肽表达促进小鼠肠道炎症。
Gastroenterology. 2019 Mar;156(4):1098-1111. doi: 10.1053/j.gastro.2018.11.033. Epub 2018 Nov 16.
6
A DNA-Methylated Sight on Autoimmune Inflammation Network across RA, pSS, and SLE.自身免疫性炎症网络中的 DNA 甲基化观察：RA、pSS 和 SLE 中的自身免疫性炎症网络。
J Immunol Res. 2018 Aug 12;2018:4390789. doi: 10.1155/2018/4390789. eCollection 2018.
7
Evolution of ARDS biomarkers: Will metabolomics be the answer?急性呼吸窘迫综合征生物标志物的研究进展：代谢组学能否提供答案？
Am J Physiol Lung Cell Mol Physiol. 2018 Oct 1;315(4):L526-L534. doi: 10.1152/ajplung.00074.2018. Epub 2018 Jun 28.
8
Author Correction: Analysis of DNA methylation in cancer: location revisited.作者更正：癌症中DNA甲基化分析：位置再探讨。
Nat Rev Clin Oncol. 2018 Jul;15(7):467. doi: 10.1038/s41571-018-0028-9.
9
Effect of TLR4/MyD88 signaling pathway on sepsis-associated acute respiratory distress syndrome in rats, via regulation of macrophage activation and inflammatory response.TLR4/MyD88信号通路通过调节巨噬细胞活化和炎症反应对大鼠脓毒症相关急性呼吸窘迫综合征的影响
Exp Ther Med. 2018 Apr;15(4):3376-3384. doi: 10.3892/etm.2018.5815. Epub 2018 Jan 30.
10
RNA-Seq analysis of peripheral blood mononuclear cells reveals unique transcriptional signatures associated with disease progression in dengue patients.登革热患者外周血单个核细胞的RNA测序分析揭示了与疾病进展相关的独特转录特征。
Transl Res. 2017 Aug;186:62-78.e9. doi: 10.1016/j.trsl.2017.06.007. Epub 2017 Jun 17.