Pérez-Maceda B, Bernabeu C, López-Bote J P, Marquet A, Larraga V
Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Scand J Immunol. 1988 Mar;27(3):295-304. doi: 10.1111/j.1365-3083.1988.tb02350.x.
We have found autoantibodies in the sera from rheumatoid arthritis (RA) patients which recognize two cell surface antigens of approximately 70 kDa and 28 kDa from synoviocyte extracts as detected by immunoprecipitation analysis. These polypeptides were immunoprecipitated from extracts containing mainly macrophage-like synoviocytes (type A) but not from extracts of homogeneous fibroblast-like synoviocytes (type B). These autoantigens are not selectively expressed by RA synoviocytes, since both RA and non-rheumatoid synovia were reactive for RA sera. From the panel of different RA sera tested, 64% immunoprecipitated the 70 kDa band, and 27% recognized the 28 kDa polypeptide. These differences in the specificity of the sera seemed to be related to the clinical state of the donor. The sera from patients suffering from other autoimmune diseases such as autoimmune thyroiditis and systemic lupus erythematosus (SLE) do not appear to be reactive for these specificities, but sera from patients with Sjögren's syndrome, psoriatic arthritis, and Crohn's disease showed a weak cross-reactivity with the 70 kDa polypeptide. This autoreactivity against synovial cells in RA supports the idea that these cells participate in the initial immune response of the disease.
我们在类风湿性关节炎（RA）患者的血清中发现了自身抗体，通过免疫沉淀分析检测到这些抗体可识别来自滑膜细胞提取物中两种分子量约为70 kDa和28 kDa的细胞表面抗原。这些多肽是从主要含有巨噬细胞样滑膜细胞（A型）的提取物中免疫沉淀出来的，而不是从均匀的成纤维细胞样滑膜细胞（B型）的提取物中免疫沉淀出来的。这些自身抗原并非RA滑膜细胞选择性表达，因为RA和非类风湿性滑膜对RA血清均有反应。在所检测的不同RA血清中，64%免疫沉淀出70 kDa条带，27%识别28 kDa多肽。血清特异性的这些差异似乎与供体的临床状态有关。患有其他自身免疫性疾病如自身免疫性甲状腺炎和系统性红斑狼疮（SLE）的患者血清对这些特异性似乎无反应，但干燥综合征、银屑病关节炎和克罗恩病患者的血清与70 kDa多肽表现出弱交叉反应。RA中针对滑膜细胞的这种自身反应性支持了这些细胞参与该疾病初始免疫反应的观点。
