从类风湿性关节炎关节建立的CD4 +、CD8 - T细胞克隆对类风湿性关节炎滑膜抗原的识别

Recognition of rheumatoid arthritis synovial antigen by CD4+,CD8- T cell clones established from rheumatoid arthritis joints.

作者信息

Toyosaki T, Tsuruta Y, Yoshioka T, Takemoto H, Suzuki R, Tomita T, Ochi T

机构信息

Shionogi Research Laboratories, Shionogi & Company, Ltd., Osaka, Japan.

出版信息

Arthritis Rheum. 1998 Jan;41(1):92-100. doi: 10.1002/1529-0131(199801)41:1<92::AID-ART12>3.0.CO;2-2.

DOI:10.1002/1529-0131(199801)41:1<92::AID-ART12>3.0.CO;2-2

PMID:9433874

Abstract

OBJECTIVE

To investigate the rheumatoid arthritis (RA)-specific autoantigen(s) recognized by CD4+ T cells in patients with RA.

METHODS

CD4+,CD45RO+ T cell clones were established from the joints of RA patients, and were examined for their proliferative response to synovial cells.

RESULTS

Eight of 146 T cell clones responded to RA synovial cells in a DR-restricted manner. These T cell clones recognized solubilized antigens extracted from RA synovial cells in the presence of DR-matched antigen-presenting cells, but did not respond to those extracted from non-RA synovial cells. The antigens had a molecular weight of 50/25 kd. Five of the 8 T cell clones used T cell receptor BV6, and the remaining clones used BV12.2.

CONCLUSION

The antigens recognized by joint-infiltrating CD4+ T cells are present exclusively in RA synovial cells. The expression of these antigens by synovial cells may trigger the autoreactivity of T cells in RA joints.

摘要

目的

研究类风湿关节炎（RA）患者中CD4⁺ T细胞识别的RA特异性自身抗原。

方法

从RA患者关节中建立CD4⁺、CD45RO⁺ T细胞克隆，并检测其对滑膜细胞的增殖反应。

结果

146个T细胞克隆中有8个以DR限制的方式对RA滑膜细胞作出反应。这些T细胞克隆在存在DR匹配的抗原呈递细胞的情况下识别从RA滑膜细胞中提取的可溶性抗原，但对从非RA滑膜细胞中提取的抗原无反应。这些抗原的分子量为50/25 kd。8个T细胞克隆中有5个使用T细胞受体BV6，其余克隆使用BV12.2。