Bläss S, Specker C, Lakomek H J, Schneider E M, Schwochau M
Institute for Genetics, University of Düsseldorf, Germany.
Ann Rheum Dis. 1995 May;54(5):355-60. doi: 10.1136/ard.54.5.355.
To improve the understanding of the pathogenesis of rheumatoid arthritis (RA) by identifying novel, disease specific autoantibodies.
Total protein preparations from synovial membranes were separated electrophoretically and immunoblotted. Sera from RA patients were screened for predominant immunoreactions by blotting. A 68 kDa antigen target of the most predominant reaction was detected and further characterised.
The dominant immunoreaction in most of the RA sera tested was with a 68 kDa antigen. The antigen is probably ubiquitously expressed. It has an isoelectric point of 5.1, is O-glycosylated, and is located in the endoplasmic reticulum, the cytoplasm, or both. Antibodies to the 68 kDa autoantigen were present in 64% of 167 RA patients tested, and could also be detected in seronegative RA patients, but were present in only 1% of 98 patients with other rheumatic diseases. They could not be detected in 55 healthy controls.
Because of its high sensitivity (64%) and specificity (99%), the anti-68 kDa autoantibody not only provides another valuable parameter for diagnosis, but also represents an antibody that may be involved in the pathological mechanisms leading to RA. This hypothesis can be tested by investigating if 68 kDa specific T cells are present in RA patients.
通过鉴定新的、疾病特异性自身抗体来增进对类风湿关节炎（RA）发病机制的理解。
滑膜总蛋白制剂经电泳分离和免疫印迹分析。通过印迹法筛选RA患者血清中的主要免疫反应。检测到最主要反应的一个68 kDa抗原靶点并进一步进行表征。
在大多数检测的RA血清中，主要免疫反应针对一个68 kDa抗原。该抗原可能广泛表达。其等电点为5.1，为O-糖基化，位于内质网、细胞质或两者之中。在167例检测的RA患者中，64%存在针对68 kDa自身抗原的抗体，血清阴性的RA患者中也能检测到，但98例其他风湿性疾病患者中只有1%存在该抗体。55名健康对照中未检测到。
由于抗68 kDa自身抗体具有高敏感性（64%）和特异性（99%），它不仅为诊断提供了另一个有价值的参数，还代表了一种可能参与导致RA的病理机制的抗体。这一假设可通过研究RA患者中是否存在68 kDa特异性T细胞来验证。
