Gong Peipan, Chen Defeng, Wang Chao, Li Mengyi, Li Xinan, Zhang Yunhui, Li Xiangrui, Zhu Xun
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
College of Plant Protection, Shenyang Agricultural University, Shenyang, China.
Front Physiol. 2021 Jan 18;11:623612. doi: 10.3389/fphys.2020.623612. eCollection 2020.
(Fabricius), (Linnaeus), (Rondani), and (Walker) (Hemiptera: Aphididae) are important pests of wheat and other cereals worldwide. In this study, the susceptibilities of four wheat aphid species to seven insecticides were assessed. Furthermore, the activities of carboxylesterase (CarE), glutathione S-transferase (GSTs), and cytochrome P450 monooxygenase (P450s) were determined in imidacloprid treated and untreated aphids. The results showed that the susceptibilities of four wheat aphid species to tested insecticides are different and has shown higher tolerance to most insecticides. Relatively higher CarE and GST activities were observed in , and P450s activities increased significantly in response to imidacloprid treatment. Moreover, susceptibility to imidacloprid were increased by the oxidase inhibitor piperonyl butoxide in (20-fold). The results we have obtained imply that P450s may play an important role in imidacloprid metabolic process in We suggest that a highly species-specific approach is essential for managing .
法布里丘斯蚜（Fabricius）、麦长管蚜（Linnaeus）、黍蚜（Rondani）和二叉蚜（Walker）（半翅目：蚜科）是全球小麦和其他谷物的重要害虫。在本研究中，评估了四种麦蚜对七种杀虫剂的敏感性。此外，还测定了吡虫啉处理和未处理蚜虫体内羧酸酯酶（CarE）、谷胱甘肽S-转移酶（GSTs）和细胞色素P450单加氧酶（P450s）的活性。结果表明，四种麦蚜对所测试杀虫剂的敏感性不同，黍蚜对大多数杀虫剂表现出较高的耐受性。在黍蚜中观察到相对较高的CarE和GST活性，并且P450s活性在吡虫啉处理后显著增加。此外，氧化酶抑制剂胡椒基丁醚使黍蚜对吡虫啉的敏感性提高了20倍。我们获得的结果表明，P450s可能在黍蚜对吡虫啉的代谢过程中起重要作用。我们建议，采用高度物种特异性的方法对于治理黍蚜至关重要。
