Unitat de Microbiologia, Departament de Ciènces Médiques Bàsiques, Facultat de Medicina I Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus 43201, Spain.
Syst Appl Microbiol. 2013 May;36(3):171-6. doi: 10.1016/j.syapm.2012.12.007. Epub 2013 Feb 26.
Previous studies indicate that Aeromonas aquariorum and Aeromonas hydrophila subsp. dhakensis are the same taxon and suggest that they should be synonymized. Using a polyphasic approach, the phenotypic and phylogenetic relationship of A. aquariorum with the 3 defined A. hydrophila subspecies (i.e. dhakensis, hydrophila, ranae) was investigated. Phylogenetic trees derived from the 16S rRNA, rpoD or gyrB genes and a multilocus phylogenetic analysis (with the concatenated sequences of gyrB, rpoD, recA, dnaJ and gyrA) confirmed that both A. aquariorum and A. hydrophila subsp. dhakensis are a unique taxon, different from the other A. hydrophila subspecies, corroborating the phenotypic and DNA-DNA hybridization (DDH) results. A formal synonymization of A. aquariorum and A. hydrophila subsp. dhakensis and a reclassification of both as Aeromonas dhakensis sp. nov. comb nov. is therefore proposed.
先前的研究表明，嗜水气单胞菌和气单胞菌亚种 dhakensis 属于同一分类群，建议将它们视为同义词。本研究采用多相方法，研究了气单胞菌亚种 dhakensis、嗜水气单胞菌和蛙气单胞菌（即 dhakensis、hydrophila、ranae）与气单胞菌亚种 aquariorum 的表型和系统发育关系。基于 16S rRNA、rpoD 或 gyrB 基因构建的系统发育树和多位点系统发育分析（gyrB、rpoD、recA、dnaJ 和 gyrA 基因的串联序列）证实，气单胞菌亚种 aquariorum 和 dhakensis 都是一个独特的分类群，与其他嗜水气单胞菌亚种不同，这与表型和 DNA-DNA 杂交（DDH）结果一致。因此，建议将气单胞菌亚种 aquariorum 和 dhakensis 正式同义词化，并将两者重新分类为德雷克气单胞菌 dhakensis sp. nov. comb nov.。
