Cao Bangrong, Zhang Liping, Liu Huifen, Ma Shiqi, Mi Kun
Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Cancer Center, School of Medicine, Sichuan Cancer Hospital and Institute, University of Electronic Science and Technology of China, Chengdu, China.
Department of Clinical Laboratory, Sichuan Provincial Maternity and Child Health Care Hospital, The Affiliated Women's and Children's Hospital of Chengdu Medical College, Chengdu, China.
Front Genet. 2021 Jan 18;11:607479. doi: 10.3389/fgene.2020.607479. eCollection 2020.
The coronavirus disease 2019 (COVID-19) pandemic, induced by the pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread rapidly all over the world. There is considerable variability among neonates, children, and adults in the incidence of infection and severe disease following exposure to SARS-CoV-2. In our study, we analyzed the transcriptome data of primate animal model of Rhesus monkeys to evaluate the expression levels of possible SARS-CoV-2 receptors and proteases and immunologic features in the lungs, colons, livers, and brains at different developmental stages. Our results revealed that and were highly expressed in neonates compared with other populations, which imply the high incidence of infection. Other potential receptors and Type II transmembrane serine proteases (TTSPs) and cathepsin of endosomal proteases also exhibited dynamic and differential expression patterns. The expression of receptors (, , and ) and proteases (, , , and ) were highly correlated during lung development, suggesting the high susceptibility of the lungs. was specifically highly expressed in the lungs and reached the highest level in neonates, similar to . Moreover, the immune cell infiltration analysis revealed immunity immaturity in neonates, implying the association with the mild or moderate type of COVID-19. The results might help researchers design protective and therapeutic strategies for COVID-19 in populations at different ages.
由致病性严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的2019冠状病毒病（COVID-19）大流行已在全球迅速蔓延。新生儿、儿童和成人在接触SARS-CoV-2后的感染发生率和严重疾病发生率存在很大差异。在我们的研究中，我们分析了恒河猴灵长类动物模型的转录组数据，以评估不同发育阶段肺、结肠、肝脏和大脑中可能的SARS-CoV-2受体、蛋白酶的表达水平以及免疫特征。我们的结果显示，与其他人群相比，[具体基因1]和[具体基因2]在新生儿中高表达，这意味着感染发生率较高。其他潜在受体、II型跨膜丝氨酸蛋白酶（TTSPs）和内体蛋白酶组织蛋白酶也表现出动态和差异表达模式。受体（[受体基因1]、[受体基因2]和[受体基因3]）和蛋白酶（[蛋白酶基因1]、[蛋白酶基因2]、[蛋白酶基因3]和[蛋白酶基因4]）在肺发育过程中的表达高度相关，表明肺的高易感性。[具体基因3]在肺中特异性高表达，并在新生儿中达到最高水平，类似于[具体基因4]。此外，免疫细胞浸润分析显示新生儿免疫不成熟，这意味着与COVID-19的轻度或中度类型有关。这些结果可能有助于研究人员为不同年龄段人群设计针对COVID-19的保护和治疗策略。
