Chang C S, Kokontis J, Liao S T
Ben May Institute, University of Chicago, IL 60637.
Science. 1988 Apr 15;240(4850):324-6. doi: 10.1126/science.3353726.
Complementary DNAs (cDNAs) encoding androgen receptors were obtained from human testis and rat ventral prostate cDNA libraries. The amino acid sequence deduced from the nucleotide sequences of the cDNAs indicated the presence of a cysteine-rich DNA-binding domain that is highly conserved in all steroid receptors. The human cDNA was transcribed and the RNA product was translated in cell-free systems to yield a 76-kilodalton protein. The protein was immunoprecipitable by human autoimmune antibodies to the androgen receptor. The protein bound androgens specifically and with high affinity.
从人睾丸和大鼠腹侧前列腺cDNA文库中获得了编码雄激素受体的互补DNA（cDNA）。从cDNA的核苷酸序列推导的氨基酸序列表明存在一个富含半胱氨酸的DNA结合结构域，该结构域在所有类固醇受体中高度保守。人cDNA被转录，RNA产物在无细胞系统中翻译，产生一种76千道尔顿的蛋白质。该蛋白质可被针对雄激素受体的人自身免疫抗体免疫沉淀。该蛋白质特异性且高亲和力地结合雄激素。
