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传统中草药白英(茄科)叶绿体全基因组的特征分析

Characterization of the complete chloroplast genome of traditional Chinese herb, Nakai. (Solanaceae).

作者信息

Zhang Xiaoxia, Zhang Xiaofeng, Wang Huan, Chen Shilong, Cheng Tingfeng, Shi Shengbo, Zhou Dangwei

机构信息

College of Medicine, Xi'an International University, Xi'an, P. R. China.

Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), NorthwestInstitute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.

出版信息

Mitochondrial DNA B Resour. 2021 Jan 19;6(1):211-213. doi: 10.1080/23802359.2020.1860713.

Abstract

Nakai. (Solanaceae) is a traditional Chinese medicine and animal fodder in Asian continent. Here, the complete chloroplast genome sequence of was determined by the Illumina Hiseq technology. The complete chloroplast genome of was 155,415 bp and the GC content was 37.81%. The typical circular quadripartite structure was composed with two inverted repeat (IR) regions with 25,588 bp, a large single-copy (LSC) region (85,931 bp) and a small single-copy (SSC) region (18,344 bp). The chloroplast genome of contained 132 unigenes, which contained 86 protein-coding genes, 37 tRNA and 8 rRNA genes and one pseudo-gene. Moreover, 183 SSRs were identified and 65% (119)of them located at LSC region. A Maximum-Likelihood (ML) phylogenetic analysis based on chloroplast genomes indicated that was closely related to , . Our results would provide a valuable resource for resource utilization and the phylogenetic studies of species of Dulcamara sect. in Solanaceae.

摘要

龙葵(茄科)是亚洲大陆的一种传统中药和动物饲料。在此,通过Illumina Hiseq技术测定了龙葵的完整叶绿体基因组序列。龙葵的完整叶绿体基因组为155,415 bp,GC含量为37.81%。典型的环状四分体结构由两个25,588 bp的反向重复(IR)区域、一个85,931 bp的大单拷贝(LSC)区域和一个18,344 bp的小单拷贝(SSC)区域组成。龙葵的叶绿体基因组包含132个单基因,其中包括86个蛋白质编码基因、37个tRNA和8个rRNA基因以及一个假基因。此外,鉴定出183个简单序列重复(SSR),其中65%(119个)位于LSC区域。基于叶绿体基因组的最大似然(ML)系统发育分析表明,龙葵与其他几种植物关系密切。我们的结果将为茄科白英组物种的资源利用和系统发育研究提供有价值的资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3324/7832539/3432aa8baf92/TMDN_A_1860713_F0001_C.jpg

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