Augusto João B, Menacho Katia, Andiapen Mervyn, Bowles Ruth, Burton Maudrian, Welch Sophie, Bhuva Anish N, Seraphim Andreas, Pade Corinna, Joy George, Jensen Melanie, Davies Rhodri H, Captur Gabriella, Fontana Marianna, Montgomery Hugh, O'Brien Ben, Hingorani Aroon D, Cutino-Moguel Teresa, McKnight Áine, Abbass Hakam, Alfarih Mashael, Alldis Zoe, Baca Georgina L, Boulter Alex, Bracken Olivia V, Bullock Natalie, Champion Nicola, Chan Carmen, Couto-Parada Xose, Dieobi-Anene Keenan, Feehan Karen, Figtree Gemma, Figtree Melanie C, Finlay Malcolm, Forooghi Nasim, Gibbons Joseph M, Griffiths Peter, Hamblin Matt, Howes Lee, Itua Ivie, Jones Meleri, Jardim Victor, Kapil Vikas, Jason Lee Wing-Yiu, Mandadapu Vineela, Mfuko Celina, Mitchelmore Oliver, Palma Susana, Patel Kush, Petersen Steffen E, Piniera Brian, Raine Rosalind, Rapala Alicja, Richards Amy, Sambile Genine, Couto de Sousa Jorge, Sugimoto Michelle, Thornton George D, Artico Jessica, Zahedi Dan, Parker Ruth, Robathan Mathew, Hickling Lauren M, Ntusi Ntobeko, Semper Amanda, Brooks Tim, Jones Jessica, Tucker Art, Veerapen Jessry, Vijayakumar Mohit, Wodehouse Theresa, Wynne Lucinda, Treibel Thomas A, Noursadeghi Mahdad, Manisty Charlotte, Moon James C
Institute of Cardiovascular Science, University College London, London, UK.
Barts Heart Centre, St Bartholomew's Hospital, Barts Health NHS Trust, London, UK, London, UK.
Wellcome Open Res. 2020 Oct 12;5:179. doi: 10.12688/wellcomeopenres.16051.2. eCollection 2020.
: Most biomedical research has focused on sampling COVID-19 patients presenting to hospital with advanced disease, with less focus on the asymptomatic or paucisymptomatic. We established a bioresource with serial sampling of health care workers (HCWs) designed to obtain samples before and during mainly mild disease, with follow-up sampling to evaluate the quality and duration of immune memory. : We conducted a prospective study on HCWs from three hospital sites in London, initially at a single centre (recruited just prior to first peak community transmission in London), but then extended to multiple sites 3 weeks later (recruitment still ongoing, target n=1,000). Asymptomatic participants attending work complete a health questionnaire, and provide a nasal swab (for SARS-CoV-2 RNA by RT-PCR tests) and blood samples (mononuclear cells, serum, plasma, RNA and DNA are biobanked) at 16 weekly study visits, and at 6 and 12 months. : Preliminary baseline results for the first 731 HCWs (400 single-centre, 331 multicentre extension) are presented. Mean age was 38±11 years; 67% are female, 31% nurses, 20% doctors, and 19% work in intensive care units. COVID-19-associated risk factors were: 37% black, Asian or minority ethnicities; 18% smokers; 13% obesity; 11% asthma; 7% hypertension and 2% diabetes mellitus. At baseline, 41% reported symptoms in the preceding 2 weeks. Preliminary test results from the initial cohort (n=400) are available: PCR at baseline for SARS-CoV-2 was positive in 28 of 396 (7.1%, 95% CI 4.9-10.0%) and 15 of 385 (3.9%, 2.4-6.3%) had circulating IgG antibodies. : This COVID-19 bioresource established just before the peak of infections in the UK will provide longitudinal assessments of incident infection and immune responses in HCWs through the natural time course of disease and convalescence. The samples and data from this bioresource are available to academic collaborators by application https://covid-consortium.com/application-for-samples/.
大多数生物医学研究都集中在对因病情严重而住院的新冠患者进行采样，较少关注无症状或症状轻微的患者。我们建立了一个生物资源库，对医护人员进行系列采样，旨在在主要为轻症的疾病发生之前和期间获取样本，并进行后续采样以评估免疫记忆的质量和持续时间。
我们对伦敦三个医院地点的医护人员进行了一项前瞻性研究，最初在一个中心（在伦敦社区传播首次高峰之前招募），但在3周后扩展到多个地点（招募仍在进行中，目标人数为1000人）。无症状的在职参与者要完成一份健康问卷，并在每周16次的研究访视中以及在6个月和12个月时提供鼻拭子（用于通过逆转录聚合酶链反应检测新冠病毒核糖核酸）和血液样本（单核细胞、血清、血浆、核糖核酸和脱氧核糖核酸被存入生物样本库）。
呈现了前731名医护人员（400名单中心、331名多中心扩展人员）的初步基线结果。平均年龄为38±11岁；67%为女性，31%为护士，20%为医生，19%在重症监护病房工作。与新冠相关的风险因素包括：37%为黑人、亚洲人或少数族裔；18%为吸烟者；13%肥胖；11%患有哮喘；7%患有高血压；2%患有糖尿病。在基线时，41%的人报告在之前两周出现过症状。初始队列（n = 400）的初步检测结果如下：在396人中，28人（7.1%，95%置信区间4.9 - 10.0%）的新冠病毒核糖核酸基线聚合酶链反应检测呈阳性，385人中15人（3.9%，2.4 - 6.3%）有循环免疫球蛋白G抗体。
这个在英国感染高峰之前建立的新冠生物资源库将通过疾病和康复的自然时间进程，对医护人员的新发感染和免疫反应进行纵向评估。通过申请 https://covid-consortium.com/application-for-samples/，该生物资源库的样本和数据可供学术合作者使用。
