Department of Clinical Laboratory, Qingdao Municipal Hospital, Qingdao, Shandong, People's Republic of China.
Department of Clinical Laboratory, Qingdao Women and Children's Hospital, Qingdao, Shandong, People's Republic of China.
Biotechnol Appl Biochem. 2022 Feb;69(1):376-382. doi: 10.1002/bab.2116. Epub 2021 Feb 26.
Thyroid cancer appears in endocrine glands and specific to thyroid glands has been reported widely. This work was targeted to identify and quantify thyroglobulin by using antithyroglobulin antibody complexed silane surface on interdigitated electrode (IDE) sensing surface. (3-Aminopropyl)triethoxysilane linker was used to make silane-coupling with antibody and attached on the hydroxylated IDE. This electroanalytical IDE revealed the dose-dependent responses with thyroglobulin concentrations. By getting increments with the thyroglobulin concentrations, the current responses were enhanced concomitantly and the thyroglobulin detection limit was noted as 1 pM on the linear curve [y = 0.1311x + 0.5386; R² = 0.9707] with the sensitivity at lower picomolar range. Moreover, the control experiments with thyroid peroxidase and nonimmune antibody cannot yield any response of current, confirming the specific detection of thyroglobulin. This research set-up is useful to determine and quantify the thyroglobulin and diagnose thyroid cancer.
甲状腺癌出现在内分泌腺中，特别是甲状腺，这已经被广泛报道。本工作旨在通过在叉指电极（IDE）传感表面上使用与抗甲状腺球蛋白抗体复合的硅烷表面来识别和定量甲状腺球蛋白。（3-氨丙基）三乙氧基硅烷连接子用于使抗体与硅烷偶联，并附着在羟基化的 IDE 上。这种电化学 IDE 揭示了与甲状腺球蛋白浓度呈剂量依赖性的响应。随着甲状腺球蛋白浓度的增加，电流响应也随之增强，在线性曲线 [y = 0.1311x + 0.5386; R² = 0.9707] 上，甲状腺球蛋白的检测限为 1 pM，灵敏度在较低的皮摩尔范围内。此外，甲状腺过氧化物酶和非免疫抗体的对照实验不能产生任何电流响应，证实了甲状腺球蛋白的特异性检测。这种研究装置可用于测定和定量甲状腺球蛋白并诊断甲状腺癌。
