Department of B-Mode Ultrasound, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial, Fuzhou, 350001, China.
Department of Nuclear Medicine, Fujian Provincial Hospital, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, China.
Anal Sci. 2023 Jun;39(6):969-975. doi: 10.1007/s44211-023-00305-9. Epub 2023 Mar 6.
As a dimeric protein, thyroglobulin (Tg) is an important biomarker for different thyroid cancer (DTC), so designing effective method to detect Tg is of great significance. In this work, by preparing β-cyclodextrin (CD) functionalized carbon nanotubes (CNTs) nanohybrid (CD-CNTs) as carrier to immobilize primary antibody (Ab) of Tg, assembling sulfydryl ferrocene (Fc) and secondary antibody (Ab) on the surface of nanogold (Au) as signaling amplifier (Ab-Au-Fc), a simple and sensitive sandwich-type electrochemical immunoassay (STEM) of Tg was designed herein for the first time. In brief, CNTs show large surface area and conductivity, while CD offers superior host-guest recognition capability that can bound with Ab; meanwhile, Fc probe can offer stable electrochemical signal that is proportionable to the concentration of Tg. Under the optimum conditions, the proposed STEM platform shows excellent sensing results for Tg detection: a considerable low analytical detection (0.5 ng mL) and wide linearity (2 to 200 ng mL), suggesting the designed STEM platform offers potential real applications for detect Tg.
作为一种二聚体蛋白，甲状腺球蛋白（Tg）是不同甲状腺癌（DTC）的重要生物标志物，因此设计有效的 Tg 检测方法具有重要意义。在这项工作中，通过制备β-环糊精（CD）功能化碳纳米管（CNTs）纳米杂化物（CD-CNTs）作为载体来固定 Tg 的一抗（Ab），将巯基二茂铁（Fc）和二抗（Ab）组装在纳米金（Au）表面作为信号放大剂（Ab-Au-Fc），首次设计了一种简单灵敏的三明治型电化学免疫分析（STEM）用于检测 Tg。简而言之，CNTs 具有较大的表面积和导电性，而 CD 具有优越的主体-客体识别能力，可以与 Ab 结合；同时，Fc 探针可以提供稳定的电化学信号，与 Tg 的浓度成正比。在最佳条件下，所提出的 STEM 平台对 Tg 检测表现出优异的传感结果：分析检测的下限（0.5ng/mL）相当低，线性范围较宽（2 至 200ng/mL），表明设计的 STEM 平台为 Tg 的检测提供了潜在的实际应用。
