Department of Medical Biochemistry, Dokuz Eylül University Faculty of Medicine, Turkey.
Department of Chest Diseases, Dokuz Eylül University Faculty of Medicine, Turkey.
Niger J Clin Pract. 2023 Jun;26(6):771-778. doi: 10.4103/njcp.njcp_737_22.
In COVID-19 patients, besides changes in leucocyte count, morphological abnormalities of circulating blood cells have been reported.
This study aims to investigate the relationship between the morphological and functional properties of leucocytes and the severity of the disease in COVID-19 patients.
Blood samples were collected from COVID-19 patients (n = 130) at the time of admission. The patients were stratified according to the comorbidity, age, LDH, lymhocyte count score as mild, moderate, and severe. Complete blood count and the cell population data were analyzed by the Volume, conductivity, scatter (VCS) technology on Beckman Coulter LH-780 hematology analyzer. Kruskal-Wal'lis test was used to assess the differences between the groups with subsequent Bonferroni correction.
Neutrophil count was increased, and lymphocyte count was decreased in severe patients compared to mild patients. The increase in the percent of neutrophils and the neutrophil/lymphocyte ratio in the severe patient group was significant in comparison to both the moderate and the mild group. The dispersion of the neutrophil volume and conductivity showed significant changes depending on the severity of the disease. The lymphocyte volume, lymphocyte-volume-SD and lymphocyte-conductivity as well as the monocyte-volume and monocyte-volume-SD were significantly increased in severe patients in comparison to mild patients. The increase of lymphocyte and monocyte volume in severe patients was also significant in comparison to moderate patients.
COVID-19 infection leads to important changes in cell population data of leucocytes. The volumetric changes in lymphocytes and monocytes are related to the severity of the disease.
在 COVID-19 患者中，除了白细胞计数的变化外，还报道了循环血细胞的形态异常。
本研究旨在探讨 COVID-19 患者白细胞的形态和功能特性与疾病严重程度之间的关系。
在入院时采集了 COVID-19 患者（n=130）的血液样本。根据合并症、年龄、LDH、淋巴细胞计数评分将患者分为轻症、中症和重症。通过贝克曼库尔特 LH-780 血液分析仪的体积、传导率、散射（VCS）技术分析全血细胞计数和细胞群数据。Kruskal-Wal'lis 检验用于评估各组之间的差异，随后进行 Bonferroni 校正。
与轻症患者相比，重症患者的中性粒细胞计数增加，淋巴细胞计数减少。与中症和轻症组相比，重症患者中性粒细胞百分比和中性粒细胞/淋巴细胞比值的增加具有统计学意义。中性粒细胞体积和传导率的分散程度根据疾病的严重程度发生了显著变化。与轻症患者相比，重症患者的淋巴细胞体积、淋巴细胞体积-SD 和淋巴细胞传导率以及单核细胞体积和单核细胞体积-SD 显著增加。与中症患者相比，重症患者的淋巴细胞和单核细胞体积增加也具有统计学意义。
COVID-19 感染导致白细胞细胞群数据发生重要变化。淋巴细胞和单核细胞的体积变化与疾病的严重程度有关。
