Senik Svetlana Viktorovna, Kolker Tatiana L, Kotlova Ekaterina R, Vlasov Dmitry Yu, Shavarda Alexey L, Puzansky Roman K, Psurtseva Nadezhda V
Komarov Botanical Institute RAS, Saint-Petersburg, Russia.
Saint-Petersburg State University, Saint-Petersburg, Russia.
Curr Microbiol. 2021 Mar;78(3):961-966. doi: 10.1007/s00284-021-02349-4. Epub 2021 Feb 4.
Basidiomycete fungus Serpula lacrymans is one of the most dangerous indoor fungus causing dry rot of timber. The physiology of this fungus deserves more attention as a basis for development of methods of dry rot treatment. We observed an increase in the freezing resistance of S. lacrymans after pre-cultivation of mycelia at elevated temperatures. To examine the biochemical mechanisms underlying this phenomenon the lipid composition and metabolite profiling of mycelia subjected to freezing and thawing were investigated. An analysis is made of the growth rate and metabolism of "daughter" cultures derived from a frozen mycelia. According to the results, sphingolipids and water-soluble metabolites such as mannitol, glycerol, sugar alcohols, some amino- and organic acids are able to function as protective compounds providing a cross-resistance between heat shock and freeze-thaw stress in S. lacrymans.
担子菌纲真菌干腐菌是导致木材干腐的最危险的室内真菌之一。作为开发干腐治疗方法的基础，这种真菌的生理学值得更多关注。我们观察到，在高温下对菌丝体进行预培养后，干腐菌的抗冻性有所提高。为了研究这一现象背后的生化机制，我们对经历冻融的菌丝体的脂质组成和代谢物谱进行了研究。对源自冷冻菌丝体的“子代”培养物的生长速率和代谢进行了分析。结果表明，鞘脂和水溶性代谢物，如甘露醇、甘油、糖醇、一些氨基酸和有机酸，能够作为保护化合物，在干腐菌中提供热休克和冻融胁迫之间的交叉抗性。
