Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, China.
Dev Comp Immunol. 2021 Sep;122:104113. doi: 10.1016/j.dci.2021.104113. Epub 2021 May 9.
The innate immune system is the first line of defense against the invasion of pathogens. It can make a rapid immune response to the invading pathogenic microorganisms, thereby eliminating the invading pathogens and protecting the body from harm. microRNAs are a family of small non-coding ribonucleic acid molecules, which are important and multifunctional regulator of immune response. In this study, we studied the role of miR-132 as a key regulatory factor of IL-1β-mediated inflammation. The seed region of miR-132 can regulate gene expression by binding to the 3'UTR of IL-1β, and inhibit the expression of IL-1β at the post-transcriptional level. More importantly, miR-132 inhibits LPS-induced NF-κB signaling pathway by targeting IL-1β, thereby preventing excessive inflammatory response from causing autoimmune diseases. These results will help to better understand the complex regulatory mechanisms of teleost fishes.
先天免疫系统是抵御病原体入侵的第一道防线。它可以对入侵的病原微生物做出快速免疫反应，从而消除入侵的病原体，保护身体免受伤害。 microRNAs 是一类小的非编码核糖核酸分子，是免疫反应的重要多功能调节因子。在这项研究中，我们研究了 miR-132 作为 IL-1β 介导的炎症的关键调节因子的作用。miR-132 的种子区可以通过与 IL-1β 的 3'UTR 结合来调节基因表达，从而在转录后水平抑制 IL-1β 的表达。更重要的是，miR-132 通过靶向 IL-1β 抑制 LPS 诱导的 NF-κB 信号通路，从而防止过度的炎症反应导致自身免疫性疾病。这些结果将有助于更好地理解硬骨鱼类复杂的调控机制。
