Liu Ruijiang, Rong Guoxiang, Liu Yanghua, Huang Wei, He Dawei, Lu Rongzhu
School of Pharmacy, Jiangsu University, Zhenjiang 212013, PR China.
The People's Hospital of Danyang, Zhenjiang 212300, PR China.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111719. doi: 10.1016/j.msec.2020.111719. Epub 2020 Nov 20.
This study introduces a mesoporous magnetic nano-system for the delivery of apigenin (API). A targeted therapeutic drug delivery system was prepared based on FeO/FeO@mSiO-HA nanocomposites. Magnetic FeO/FeO heterogeneous nanoparticles were first prepared via the rapid-combustion process. The effects of solvent type, solvent volume, calcination temperature, and calcination time on the crystal size and magnetism of the FeO/FeO heterogeneous nanoparticles were investigated. The mesoporous silica shell was deposited on the FeO/FeO heterogeneous nanoparticles using an improved Stöber method. HA was exploited as the targeting ligand. The specific surface area of the FeO/FeO@mSiO nanocomposites was 369.6 m/g, which is 19 times higher than that of the magnetic FeO/FeO heterogeneous nanoparticle cores. Drug release properties from the FeO/FeO@mSiO-HA nanocomposites were studied, and the result showed that API-loaded nano-system had sustained release effect. Prussian blue staining and electrochemical performance variation showed that an external magnetic field facilitated cell uptake of FeO/FeO@mSiO-HA nanocomposites. MTT assays showed that the cell inhibition effect of API-FeO/FeO@mSiO-HA was stronger than that of free API at the same drug dose under a magnetic field and FeO/FeO@mSiO-HA nanocomposites showed good biocompatibility. Fluorescence imaging, flow cytometry, western blot, reactive oxygen species (ROS), Superoxide dismutase (SOD) and malondialdehyde (MDA) kits verified that the enhanced therapeutic action was due to the promotion of apoptosis, lipid peroxidation, and ferroptosis. The magnetic nano-system (FeO/FeO@mSiO-HA) showed good magnetic targeting and active hyaluronic acid targeting, and has the potential to provide a targeted delivery platform for many antitumor drugs.
本研究介绍了一种用于递送芹菜素（API）的介孔磁性纳米系统。基于FeO/FeO@mSiO-HA纳米复合材料制备了一种靶向治疗药物递送系统。首先通过快速燃烧法制备了磁性FeO/FeO异质纳米颗粒。研究了溶剂类型、溶剂体积、煅烧温度和煅烧时间对FeO/FeO异质纳米颗粒晶体尺寸和磁性的影响。采用改进的Stöber法在FeO/FeO异质纳米颗粒上沉积介孔二氧化硅壳。HA被用作靶向配体。FeO/FeO@mSiO纳米复合材料的比表面积为369.6 m/g，比磁性FeO/FeO异质纳米颗粒核高19倍。研究了FeO/FeO@mSiO-HA纳米复合材料的药物释放性能，结果表明载有API的纳米系统具有缓释效果。普鲁士蓝染色和电化学性能变化表明，外部磁场促进了FeO/FeO@mSiO-HA纳米复合材料的细胞摄取。MTT试验表明，在相同药物剂量下，磁场作用下API-FeO/FeO@mSiO-HA的细胞抑制作用强于游离API，且FeO/FeO@mSiO-HA纳米复合材料具有良好的生物相容性。荧光成像、流式细胞术、蛋白质免疫印迹、活性氧（ROS）、超氧化物歧化酶（SOD）和丙二醛（MDA）试剂盒证实，增强的治疗作用归因于细胞凋亡、脂质过氧化和铁死亡的促进。磁性纳米系统（FeO/FeO@mSiO-HA）表现出良好的磁靶向和活性透明质酸靶向性，有潜力为多种抗肿瘤药物提供靶向递送平台。
