Petralanda I, Yarzabal L, Piessens W F
Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts 02115.
Am J Trop Med Hyg. 1988 Mar;38(2):372-9. doi: 10.4269/ajtmh.1988.38.372.
We used a noncompetitive two-site ELISA with 5 monoclonal antibodies to determine whether parasite antigens are present in breast milk from women infected with Onchocerca volvulus. Seven out of 13 available milk samples contained significant amounts of filarial antigens. Antigen indices in milk correlated with levels of microfilarodermia (Rs = 0.74, P less than 0.005). Antigen-containing milk samples markedly inhibited mitogen-induced proliferation of human mononuclear cells and activated cells within this population that suppressed the proliferative response of autologous lymphocytes to mitogens and antigens. These findings indicate that parasite products are present in breast milk of O. volvulus-infected women and suggest that these may induce immune tolerance and/or suppression in infants born of infected mothers.
我们使用一种含有5种单克隆抗体的非竞争性双位点酶联免疫吸附测定法，以确定感染盘尾丝虫的妇女的母乳中是否存在寄生虫抗原。在13份可用的乳汁样本中，有7份含有大量丝虫抗原。乳汁中的抗原指数与微丝蚴血症水平相关（Rs = 0.74，P小于0.005）。含有抗原的乳汁样本显著抑制有丝分裂原诱导的人单核细胞增殖，并激活该群体中的细胞，这些细胞抑制自体淋巴细胞对有丝分裂原和抗原的增殖反应。这些发现表明，盘尾丝虫感染妇女的母乳中存在寄生虫产物，并提示这些产物可能会在受感染母亲所生婴儿中诱导免疫耐受和/或免疫抑制。
