Engineering Research Centre of Bioprocess of Ministry of Education, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China.
School of Food and Biological Engineering, Hefei University of Technology, No. 193 Tunxi Road, Hefei 230009, People's Republic of China.
J Agric Food Chem. 2023 Apr 26;71(16):6468-6479. doi: 10.1021/acs.jafc.2c08192. Epub 2023 Apr 12.
Osteocalcin was reported to regulate muscle energy metabolism, thus fighting fatigue during exercise. The current work aimed to investigate the anti-fatigue effect and the underlying mechanism of a homogeneous polysaccharide (PCPY-1) from after structure characterization. In the exhaustive swimming mouse model and the co-culture system of BMSCs/C2C12 cells, PCPY-1 significantly stimulated BMSC differentiation into osteoblasts as determined by ALP activity, matrix mineralization, and the protein expressions of osteogenic markers BMP-2, phosphor-Smad1, RUNX2, and osteocalcin. Meanwhile, PCPY-1 remarkably enhanced myoblast energy metabolism by upregulating osteocalcin release and GPRC6A protein expression; the phosphorylation levels of CREB and HSL; the mRNA levels of GLUT4, CD36, FATP1, and CPT1B; and ATP production in vitro and in vivo. Accordingly, PCPY-1 exhibited good anti-fatigue capacity in mice as confirmed by fatigue-related indicators. Our findings indicated PCPY-1 could enhance osteocalcin-mediated communication between bones and muscles, which was conducive to muscle energy metabolism and ATP generation, thus alleviating fatigue in exhausted swimming mice.
骨钙素被报道能调节肌肉能量代谢，从而在运动中对抗疲劳。本工作旨在研究一种均一多糖（PCPY-1）的抗疲劳作用及其潜在机制。在力竭游泳小鼠模型和 BMSCs/C2C12 细胞共培养体系中，PCPY-1 显著促进 BMSC 向成骨细胞分化，通过碱性磷酸酶活性、基质矿化以及骨形成标志物 BMP-2、磷酸化 Smad1、RUNX2 和骨钙素的蛋白表达来确定。同时，PCPY-1 通过上调骨钙素释放和 GPRC6A 蛋白表达、CREB 和 HSL 的磷酸化水平、GLUT4、CD36、FATP1 和 CPT1B 的 mRNA 水平以及体外和体内的 ATP 生成，显著增强肌细胞的能量代谢。因此，PCPY-1 通过疲劳相关指标证实了其在小鼠中的良好抗疲劳能力。我们的研究结果表明，PCPY-1 可以增强骨骼和肌肉之间的骨钙素介导的通讯，有利于肌肉能量代谢和 ATP 的产生，从而缓解力竭游泳小鼠的疲劳。
