Sacks J M, Gillette K G, Frank G H
US Department of Agriculture, National Animal Disease Center, Ames, LA 50010.
Am J Vet Res. 1988 Jan;49(1):38-41.
A 2-stage strategy was developed and evaluated for estimating serum antibody titer by use of ELISA and a series of dilutions. In stage 1, the linear response region and least-square estimate of the assay line slope were established from 9-point dilution assays. Provided that the reading was within the linear response region, this information was used in the stage-2 estimation of titer from a single absorbance reading. Operationally, 2 fixed dilutions were selected, one suitably low and one suitably high, to provide at least one reading within the linear region. The procedure should save considerable time when a large number of assays are to be performed. Stage 1 required approximately twenty 9-point assays, but all subsequent assays required only 2 fixed dilutions.
我们开发并评估了一种两阶段策略，用于通过酶联免疫吸附测定（ELISA）和一系列稀释来估计血清抗体滴度。在第一阶段，通过9点稀释测定确定测定线斜率的线性响应区域和最小二乘估计值。如果读数在该线性响应区域内，则在第二阶段根据单次吸光度读数估计滴度时使用此信息。在实际操作中，选择两种固定稀释度，一种适当低，一种适当高，以确保至少有一个读数在线性区域内。当需要进行大量测定时，该方法应能节省大量时间。第一阶段大约需要进行二十次9点测定，但所有后续测定仅需两种固定稀释度。
