Nagpal Megha, Taneja Sonali, Kumar Mohit, Dudeja Chetna
Private Practitioner, New Delhi, India.
Department of Conservative Dentistry & Endodontics, ITS Dental College and Hospital, Ghaziabad, Uttar Pradesh, India.
J Conserv Dent. 2020 May-Jun;23(3):221-226. doi: 10.4103/JCD.JCD_91_20. Epub 2020 Dec 4.
This study investigated the effect of various synthetic (galardin [Gal] and benzalkonium chloride [BAC]) and natural agents (hesperidin [HES] and epigallocatechin gallate) on the stability of dentin collagen matrix to resist collagenase degradation and improve long-term microtensile bond strength.
Ten sound-impacted third molars were collected and manual removal of pulp, periodontal ligament, cementum, and enamel was done. Remaining dentin fragments were pulverized under liquid nitrogen to obtain dentin powder. 2 mg aliquot of dentin powder was allocated to each of the test solutions and subjected to hydroxyproline assay. Another 60 sound human third molars were collected and occlusal enamel was ground flat to reach dentinoenamel junction. Class I cavities were prepared in dentin, followed by etching using 37% phosphoric acid for 15 s. Samples were then subjected to surface treatment with different agents for 60 s, followed by application of Optibond S and restoration with P 60 composite resin. Samples of all groups except control were subject to thermocycling. Samples were sectioned to 1 mm thick slabs which were subject to universal testing machine to determine ultimate tensile strength. One-way analysis of variance and Bonferroni test with a significance level of < 0.05 were used to analyze data.
HES resulted in maximum resistance to collagen degradation, followed by epigallocatechin gallate (EGCG), Gal, and BAC with a significant difference among the groups. Samples of Gal group showed the highest microtensile bond strength values, followed by HES, EGCG, BAC with a significant difference between the groups except HES and EGCG where the difference was nonsignificant.
The use of matrix metalloproteinase silencers could improve the mechanical properties of collagen and resist enzymatic degradation, leading to an improved long-term intimate restoration.
本研究调查了多种合成剂（加拉定[Gal]和苯扎氯铵[BAC]）以及天然剂（橙皮苷[HES]和表没食子儿茶素没食子酸酯）对牙本质胶原基质稳定性的影响，以抵抗胶原酶降解并提高长期微拉伸粘结强度。
收集10颗健全的阻生第三磨牙，手动去除牙髓、牙周膜、牙骨质和牙釉质。将剩余的牙本质碎片在液氮下粉碎以获得牙本质粉末。将2毫克的牙本质粉末等分试样分配到每种测试溶液中，并进行羟脯氨酸测定。另外收集60颗健全的人类第三磨牙，将咬合面牙釉质磨平至牙本质釉质交界处。在牙本质中制备I类洞，然后用37%磷酸蚀刻15秒。然后用不同的试剂对样品进行60秒的表面处理，接着应用Optibond S并用P 60复合树脂进行修复。除对照组外，所有组的样品均进行热循环。将样品切成1毫米厚的薄片，用万能试验机测定其极限拉伸强度。采用显著性水平<0.05的单因素方差分析和Bonferroni检验进行数据分析。
HES对胶原降解的抵抗作用最大，其次是表没食子儿茶素没食子酸酯（EGCG）、Gal和BAC，各组之间存在显著差异。Gal组的样品显示出最高的微拉伸粘结强度值，其次是HES、EGCG、BAC，除HES和EGCG组之间差异不显著外，各组之间存在显著差异。
使用基质金属蛋白酶抑制剂可以改善胶原的机械性能并抵抗酶降解，从而实现更好的长期紧密修复。
