Li Pei-Fei, Zhang Zhi-Xin, Yuan Xin, Song Hao-Jun, Ding Xiao-Yun, Xu Lei
Department of Gastroenterology, Ningbo First Hospital, Ningbo, Zhejiang 315010, P.R. China.
College of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
Oncol Lett. 2021 Feb;21(2):154. doi: 10.3892/ol.2020.12415. Epub 2020 Dec 31.
Circular RNAs (circRNAs) are a novel class of endogenous non-coding RNA molecules that are extensively expressed in a variety of species. Recently, increasing evidence suggests that circRNAs have vital functions indifferent types of human cancer, such as gastric cancer, papillary thyroid cancer and lung cancer. However, the roles of circRNAs in the development of colorectal cancer (CRC) remain unclear. The present study aimed to determine the molecular mechanism underlying hsa_circ_0001696 on the proliferation and migration of CRC cells. Reverse transcription-quantitative PCR analysis was performed to detect hsa_circ_0001696 expression in 18 paired CRC tissues and matched adjacent normal tissues. RNA interference was also performed to decrease hsa_circ_0001696 expression, and its biological effects were further assessed via flow cytometry, wound healing, colony formation and western blot assays. The results demonstrated that hsa_circ_0001696 expression was significantly lower in CRC tissues compared with adjacent normal tissues. Furthermore, hsa_circ_0001696 knockdown promoted cell proliferation and migration, and the number of cell colonies significantly increased. In addition, western blot analysis demonstrated that the protein expression levels of cyclin-dependent kinase 4 (CDK4), cyclin D, cyclin E and matrix metalloproteinase 9 (MMP9) increased. Taken together, the results of the present study demonstrated that hsa_circ_0001696 expression was downregulated in CRC tissues, and inhibition of hsa_circ_0001696 promoted cell proliferation and migration by regulating the levels of CDK4, cyclin D, cyclin E and MMP9.
环状RNA（circRNAs）是一类新型的内源性非编码RNA分子，在多种物种中广泛表达。最近，越来越多的证据表明，circRNAs在不同类型的人类癌症中具有重要功能，如胃癌、甲状腺乳头状癌和肺癌。然而，circRNAs在结直肠癌（CRC）发生发展中的作用仍不清楚。本研究旨在确定hsa_circ_0001696对CRC细胞增殖和迁移的分子机制。采用逆转录定量PCR分析检测18对CRC组织及其配对的相邻正常组织中hsa_circ_0001696的表达。还进行了RNA干扰以降低hsa_circ_0001696的表达，并通过流式细胞术、伤口愈合、集落形成和蛋白质印迹分析进一步评估其生物学效应。结果表明，与相邻正常组织相比，hsa_circ_0001696在CRC组织中的表达显著降低。此外，hsa_circ_0001696敲低促进了细胞增殖和迁移，细胞集落数量显著增加。此外，蛋白质印迹分析表明，细胞周期蛋白依赖性激酶4（CDK4）、细胞周期蛋白D、细胞周期蛋白E和基质金属蛋白酶9（MMP9）的蛋白表达水平增加。综上所述，本研究结果表明，hsa_circ_0001696在CRC组织中的表达下调，抑制hsa_circ_0001696通过调节CDK4、细胞周期蛋白D、细胞周期蛋白E和MMP9的水平促进细胞增殖和迁移。
