环状RNA hsa_circ_0044556的鉴定及其对结直肠癌进展的影响。

Identification of circular RNA hsa_circ_0044556 and its effect on the progression of colorectal cancer.

作者信息

Jing Liang, Wu Junhui, Tang Xiaocheng, Ma Min, Long Fei, Tian Buning, Lin Changwei

机构信息

Department of Gastrointestinal Surgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan China.

出版信息

Cancer Cell Int. 2020 Sep 1;20:427. doi: 10.1186/s12935-020-01523-1. eCollection 2020.

BACKGROUND

Circular RNAs (circRNAs) are a novel class of noncoding RNAs. Increasing evidence indicates that circRNAs play an important role in the occurrence and development of tumors. However, the role of circRNA hsa_circ_0044556 in the progression of colorectal cancer (CRC) remains unclear.

METHODS

First, we searched for differentially expressed circRNAs using a circRNA microarray in paired CRC and adjacent normal tissues. The circRNA hsa_circ_0044556 was screened out from the existing CRC circRNA microarray in the Gene Expression Omnibus database and our microarray. The clinical significance of hsa_circ_0044556 expression level in CRC patients was then investigated. Finally, the functions of the targets of this circRNA were determined in CRC cell lines.

RESULTS

Hsa_circ_0044556 was highly expressed in CRC patients and was positively correlated with tumor stage and lymph node metastasis. In CRC cell lines, the proliferation, migration, and invasion of cancer cells were inhibited by knocking down hsa_circ_0044556 expression.

CONCLUSION

Hsa_circ_0044556 promoted the progression of CRC. It is possible that hsa_circ_0044556 will become a novel biomarker or therapeutic target for CRC.

背景

环状RNA（circRNAs）是一类新型的非编码RNA。越来越多的证据表明，circRNAs在肿瘤的发生和发展中起重要作用。然而，circRNA hsa_circ_0044556在结直肠癌（CRC）进展中的作用仍不清楚。

方法

首先，我们使用circRNA微阵列在配对的CRC组织和相邻正常组织中寻找差异表达的circRNAs。从基因表达综合数据库中现有的CRC circRNA微阵列和我们自己的微阵列中筛选出circRNA hsa_circ_0044556。然后研究hsa_circ_0044556表达水平在CRC患者中的临床意义。最后，在CRC细胞系中确定该circRNA靶标的功能。