Isolation and characterization of a novel hydrocarbonoclastic and biosurfactant producing bacterial strain: RP3.

In this study, an indigenous novel hydrocarbonoclastic (kerosene and diesel degrading) and biosurfactant producing strain  RP3 was identified. The characteristics of bacterial strain were ascertained through its unique morphological and biochemical attributes, 16S RNA sequencing, and phylogenetic analysis. The degradation of hydrocarbons by  RP3 was observed at Day 7, Day 10 and Day 14 of the experimental duration. GC-FID chromatograms demonstrated a significant increase in hydrocarbon degradation (%) with progressing days (from 7 to 14). The bacterium exhibited capability to utilize and degrade -hexadecane (used for primary screening) and petroleum hydrocarbons (kerosene and diesel; by ≥ 90%). With increase in the number of experimentation days, the optical density of the culture medium increased, whereas pH declined (became acidic) for both Kerosene and Diesel. Absence of resistance to routinely used antibiotics makes it an ideal candidate for future field application. The study is, thus, significant in view of toxicological implications of hydrocarbons and their degradation using environmentally safe techniques so as to maintain ecological and human health.