Lodz University of Technology, Faculty of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Stefanowskiego 4/10, 90-924, Łódź, Poland.
Lodz University of Technology, Faculty of Biotechnology and Food Sciences, Institute of Molecular and Industrial Biotechnology, Stefanowskiego 4/10, 90-924, Łódź, Poland.
Chemosphere. 2020 Jul;250:126203. doi: 10.1016/j.chemosphere.2020.126203. Epub 2020 Feb 18.
Removal of slop oil, a by-product of oil refining, also obtained in cleaning up of oil tanks and filters is a difficult issue. High content of hydrocarbons (C3-C40) and other organic compounds makes this waste difficult to eliminate from the environment. The purpose of this investigation was to combine bacterial degradation by endophytic Bacillus cereus EN18 with biotransformation performed using lipase enzyme preparation (Palatase®) to remove recalcitrant compounds present in slop oil from the environment. Endophytic B. cereus EN18 was able to biodegrade up to 40% of slop oil while supplementation with lipase improved the efficiency of contamination removal in about one third. Also the use of lipase enzyme preparation resulted in higher microbial activity of B. cereus EN18 bacterial strain, as well as higher concentration of fatty acids in the culture medium, which indicates higher degradation efficiency. Obtained results suggest that lipase preparation from Rhizomucor miehei (Palatase®) may be a useful agent to improve microbial degradation of recalcitrant pollutants, like slop oil in water environments. GC and spectrometric analysis revealed that hydrocarbons from slop oil were effectively degraded while using both microbial degradation and lipase catalysis.
从环境中去除炼油副产品——污油（在清理油罐和过滤器时也会产生）是一个难题。高含量的碳氢化合物（C3-C40）和其他有机化合物使这种废物难以消除。本研究的目的是将内生芽孢杆菌 EN18 的细菌降解与使用脂肪酶制剂（Palatase®）进行的生物转化相结合，以去除污油中存在的难生物降解化合物。内生芽孢杆菌 EN18 能够生物降解高达 40%的污油，而添加脂肪酶则将污染去除效率提高了约三分之一。脂肪酶制剂的使用还导致内生芽孢杆菌 EN18 细菌菌株的微生物活性更高，培养基中脂肪酸的浓度也更高，这表明降解效率更高。研究结果表明，米赫根毛霉来源的脂肪酶制剂（Palatase®）可能是改善微生物降解水环境中难降解污染物（如污油）的有用试剂。GC 和光谱分析表明，在使用微生物降解和脂肪酶催化时，污油中的碳氢化合物被有效地降解。
