Meena Balakrishnan, Anburajan Lawrance, Vinithkumar Nambali Valsalan, Kirubagaran Ramalingam, Dharani Gopal
Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Port Blair-744103, Andaman and Nicobar Islands, India.
Marine Biotechnology Division, Ocean Science and Technology for Islands Group, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Chennai 600100, Tamil Nadu, India.
Data Brief. 2021 Jan 21;35:106727. doi: 10.1016/j.dib.2021.106727. eCollection 2021 Apr.
Trehalose, a non-reducing disaccharide (α-D-glucopyranosyl-(1→1)-α-D-glucopyranoside) is a natural compound, which serves as a protective substance in halophilic bacterial cells. Trehalose biosynthesis genes ( and ) were PCR amplified from the genomic DNA of deep sea actinobacteria, NIOT-DSA03. The amplified genes were cloned and nucleotide sequences were determined. sequence and phylogenetic analysis of nucleotides and amino acids of and sequences of were also determined. The experimental data described in this study will be helpful to develop a recombinant expression system to produce trehalose for biotechnological applications.
海藻糖是一种非还原性二糖（α-D-吡喃葡萄糖基-(1→1)-α-D-吡喃葡萄糖苷），是一种天然化合物，在嗜盐细菌细胞中作为一种保护物质。从深海放线菌NIOT-DSA03的基因组DNA中通过PCR扩增出海藻糖生物合成基因（和）。对扩增的基因进行克隆并测定核苷酸序列。还测定了和序列的核苷酸和氨基酸的序列及系统发育分析。本研究中描述的实验数据将有助于开发一种重组表达系统，以生产用于生物技术应用的海藻糖。
