Buitrago-Duque Carlos, Garcia-Sucerquia Jorge
Universidad Nacional de Colombia sede Medellín, School of Physics, A.A: 3840, Medellín 050034, Colombia.
Heliyon. 2021 Jan 30;7(1):e06098. doi: 10.1016/j.heliyon.2021.e06098. eCollection 2021 Jan.
The reduction of speckle noise by physically changing the pupil of the imaging system, as first envisioned in optical holography, is experimentally applied to a digital holographic microscope (DHM). The imaging pupil of a DHM, operating in image plane telecentric-afocal architecture, is changed in a controlled way between successive recordings, allowing the shooting of multiple partially-decorrelated holograms. Averaging the numerically reconstructed holograms yields amplitude and/or phase images with reduced speckle noise. Experimental results of biological specimens and a phase-only resolution test show the feasibility to recover micron-sized features in images with reduced speckle noise.
通过物理改变成像系统的光瞳来减少散斑噪声，这一最初在光学全息术中所设想的方法，已通过实验应用于数字全息显微镜（DHM）。在像平面远心无焦架构下运行的DHM的成像光瞳，在连续记录之间以可控方式改变，从而能够拍摄多个部分去相关的全息图。对数字重建的全息图进行平均，可得到散斑噪声降低的幅度和/或相位图像。生物样本的实验结果和仅相位分辨率测试表明，在散斑噪声降低的图像中恢复微米级特征是可行的。
