Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
Diel Laboratory, Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, USA.
Arch Virol. 2021 Apr;166(4):1163-1170. doi: 10.1007/s00705-020-04910-1. Epub 2021 Feb 7.
The envelope glycoprotein E2 of pestiviruses is a major target for neutralizing antibodies. In this study, we analyzed the E2 DA domain of 43 pestiviruses from Southern Brazil. The isolates were identified as Bovine viral diarrhea virus (BVDV) subtypes 1a and 1b or BVDV-2b. Compared to reference strains, the BVDV-1 and -2 isolates had four and two mutations in the DA domain, respectively. All BVDV-2 isolates had a deletion of residues 724 and 725. All mutated amino acids in the BVDV isolates had the same aa substitution, and all were in previously identified antibody binding sites. It is possible that an immunity-mediated selection is acting on the pestiviruses circulating in Southern Brazil.
虫媒病毒的包膜糖蛋白 E2 是中和抗体的主要靶标。本研究分析了来自巴西南部的 43 株虫媒病毒的 E2 DA 结构域。分离株被鉴定为牛病毒性腹泻病毒（BVDV）1a 和 1b 亚型或 BVDV-2b。与参考株相比，BVDV-1 和 -2 分离株在 DA 结构域分别有四个和两个突变。所有 BVDV-2 分离株都有 724 和 725 残基的缺失。BVDV 分离株中所有突变的氨基酸都有相同的 aa 取代，且都在先前鉴定的抗体结合位点内。在巴西南部流行的虫媒病毒可能正在受到免疫介导的选择。
