Department of Chemical Pathology, NSW Health Pathology, Prince of Wales Hospital, Sydney, Australia.
School of Biomedical Sciences, Charles Sturt University, Sydney, Australia.
Clin Chem Lab Med. 2020 Dec 3;59(5):947-954. doi: 10.1515/cclm-2020-1224. Print 2021 Apr 27.
Venous blood gas (VBG) analysis is becoming a popular alternative to arterial blood gas (ABG) analysis due to reduced risk of complications at phlebotomy and ease of draw. In lack of published data, this study aimed to establish reference intervals (RI) for correct interpretation of VBG results.
One hundred and 51 adult volunteers (101 females, 50 males, 18-70 years) were enrolled after completion of a health questionnaire. Venous blood was drawn into PICO syringes and analysed on ABL827 blood gas analyser (Radiometer Pacific Pty. Ltd.). A non-parametric approach was used to directly establish the VBG RI which was compared to a calculated VBG RI based on a meta-analysis of differences between ABG and VBG.
After exclusions, 134 results were used to derive VBG RI: pH 7.30-7.43, partial pressure of carbon dioxide (pCO) 38-58 mmHg, partial pressure of oxygen (pO) 19-65 mmHg, bicarbonate (HCO-) 22-30 mmol/L, sodium 135-143 mmol/L, potassium 3.6-4.5 mmol/L, chloride 101-110 mmol/L, ionised calcium 1.14-1.29 mmol/L, lactate 0.4-2.2 mmol/L, base excess (BE) -1.9-4.5 mmol/L, saturated oxygen (sO) 23-93%, carboxyhaemoglobin 0.4-1.4% and methaemoglobin 0.3-0.9%. The meta-analysis revealed differences between ABG and VBG for pH, HCO-, pCO and pO of 0.032, -1.0 mmol/L, -4.2 and 39.9 mmHg, respectively. Using this data along with established ABG RI, calculated VBG RI of pH 7.32-7.42, HCO- 23 - 27 mmol/L, pCO 36-49 mmHg (female), pCO 39-52 mmHg (male) and pO 43-68 mmHg were formulated and compared to the VBG RI of this study.
An adult reference interval has been established to assist interpretation of VBG results.
由于静脉血气（VBG）分析在采血时并发症风险较低且操作简便，因此它正逐渐成为动脉血气（ABG）分析的替代方法。由于缺乏已发表的数据，本研究旨在建立 VBG 结果正确解读的参考区间（RI）。
101 名女性和 50 名男性志愿者（年龄 18-70 岁）在完成健康问卷后被纳入研究。静脉血用 PICO 注射器采集，并使用 ABL827 血气分析仪（丹麦雷度米特公司）进行分析。本研究采用非参数方法直接建立 VBG RI，并与基于 ABG 和 VBG 差值的荟萃分析计算出的 VBG RI 进行比较。
排除后，共使用 134 份结果来推导 VBG RI：pH 值 7.30-7.43，二氧化碳分压（pCO）38-58mmHg，氧分压（pO）19-65mmHg，碳酸氢盐（HCO-）22-30mmol/L，钠 135-143mmol/L，钾 3.6-4.5mmol/L，氯 101-110mmol/L，离子钙 1.14-1.29mmol/L，乳酸 0.4-2.2mmol/L，碱剩余（BE）-1.9-4.5mmol/L，氧饱和度（sO）23-93%，碳氧血红蛋白 0.4-1.4%和高铁血红蛋白 0.3-0.9%。荟萃分析显示 ABG 和 VBG 之间的 pH 值、HCO-、pCO 和 pO 差值分别为 0.032、-1.0mmol/L、-4.2mmHg 和 39.9mmHg。使用这些数据以及已建立的 ABG RI，计算出的 VBG RI 为 pH 值 7.32-7.42、HCO- 23-27mmol/L、pCO 36-49mmHg（女性）、pCO 39-52mmHg（男性）和 pO 43-68mmHg，并与本研究的 VBG RI 进行了比较。
本研究建立了成人参考区间，以协助解读 VBG 结果。
