Hughes Jodie, Bardell David
Institute of Veterinary Science, University of Liverpool, Wirral, UK.
Institute of Veterinary Science, University of Liverpool, Wirral, UK; Institute of Ageing and Chronic Disease, Department of Musculoskeletal Biology, University of Liverpool, Liverpool, UK.
Vet Anaesth Analg. 2019 Nov;46(6):765-771. doi: 10.1016/j.vaa.2019.04.015. Epub 2019 Jun 14.
To establish reference intervals for arterial blood-gas (ABG), acid-base and electrolyte values from a healthy equine population.
Retrospective clinical study.
A total of 139 client-owned, systemically healthy horses, 1 year of age and older, presented for elective surgical procedures.
Blood samples were collected anaerobically from the transverse facial or common carotid artery of horses breathing room air, prior to administration of preanaesthetic medication. Samples were analysed immediately, without correction for body temperature, using an automated bench-top analyser. Variables analysed included pH, arterial partial pressure of carbon dioxide (PaCO) and arterial partial pressure of oxygen (PaO) and plasma concentrations of sodium (Na), potassium (K), calcium (Ca) and chloride (Cl). Actual and standardized plasma bicarbonate concentration [HCO (P) and HCO (P, st)], blood and extracellular fluid base excess [base (B) and base (ECF)] and anion gap (AG) were calculated by the machine from preprogrammed algorithms. Methods used for determination of PaCO, PaO, HCO (P), HCO (P, st), base (B) and base (ECF) met the guidelines of the Clinical and Laboratory Standards Institute. Reference intervals were determined with the nonparametric or the standard parametric method dependent on data distribution.
Reference intervals were determined for pH, 7.37-7.49; PaCO, 4.84-7.20 kPa (36.3-54.0 mmHg); PaO, 11.01-14.97 kPa (82.6-112.3 mmHg); Na, 133-141 mmol L; K, 3.05-4.65 mmol L; Ca, 1.34-1.72 mmol L; Cl, 100-110 mmol L; HCO (P), 23.55-33.90 mmol L; HCO (P, st), 23.87-32.45 mmol L; base (B), 0.51-8.80 mmol L; base (ECF), -0.53 to 9.39 mmol L and AG, 1.5-11.5 mEq L.
These data were derived from the largest group of horses reported in a single study and may aid in interpretation of ABG, acid-base and electrolyte measurements in clinical practice.
建立健康马群动脉血气（ABG）、酸碱及电解质值的参考区间。
回顾性临床研究。
共有139匹客户拥有的、全身健康的1岁及以上马匹，因择期手术前来就诊。
在给予麻醉前用药之前，从呼吸室内空气的马的横面动脉或颈总动脉无氧采集血样。使用台式自动分析仪立即对样本进行分析，无需校正体温。分析的变量包括pH值、动脉血二氧化碳分压（PaCO₂）、动脉血氧分压（PaO₂）以及血浆钠（Na⁺）、钾（K⁺）、钙（Ca²⁺）和氯（Cl⁻）浓度。机器根据预编程算法计算实际和标准化血浆碳酸氢盐浓度[HCO₃⁻(P)和HCO₃⁻(P,st)]、血液和细胞外液碱剩余[碱剩余(B)和细胞外液碱剩余(ECF)]以及阴离子间隙(AG)。用于测定PaCO₂、PaO₂、HCO₃⁻(P)、HCO₃⁻(P,st)、碱剩余(B)和细胞外液碱剩余(ECF)的方法符合临床和实验室标准协会的指南。根据数据分布，采用非参数法或标准参数法确定参考区间。
确定的参考区间为：pH值7.37 - 7.49；PaCO₂ 4.84 - 7.20 kPa（36.3 - 54.0 mmHg）；PaO₂ 11.01 - 14.97 kPa（82.6 - 112.3 mmHg）；Na⁺ 133 - 141 mmol/L；K⁺ 3.05 - 4.65 mmol/L；Ca²⁺ 1.34 - 1.72 mmol/L；Cl⁻ 100 - 110 mmol/L；HCO₃⁻(P) 23.55 - 33.90 mmol/L；HCO₃⁻(P,st) 23.87 - 32.45 mmol/L；碱剩余(B) 0.51 - 8.80 mmol/L；细胞外液碱剩余(ECF) -0.53至9.39 mmol/L；阴离子间隙(AG) 1.5 - 11.5 mEq/L。
这些数据来自单一研究中报道的最大马群样本，可能有助于临床实践中对ABG、酸碱及电解质测量结果的解读。
