Wuxi School of Medicine, Jiangnan University , Wuxi, China.
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi, China.
Virulence. 2021 Dec;12(1):679-689. doi: 10.1080/21505594.2021.1883933.
Enhancing virus multiplication could assist in the rapid production of vaccines against viral diseases. Cold atmospheric plasma (CAP), a physical approach relying on reactive oxygen species to achieve the desirable cellular outcome, was shown to be effective in enhancing virus propagation, where bovine rhinotrachieitis virus and Madin-Darby Bovine Kidney cells were used as the modeling virus and cell line, respectively. CAP was shown to create synergies with virus infection in arresting host cells at the G2/M stage, decreasing cell membrane potential, increasing intracellular calcium level, and inducing selective autophagy. In addition, CAP was demonstrated to suppress virus-triggered immunogenic signaling as evaluated by IRF7 expression. We presented evidences on CAP-triggered maximization of host resources toward virus multiplication that is advantageous for viral vaccine production, and opened a novel regime for applying CAP in the sector of medical care and health.
增强病毒繁殖能力有助于快速生产针对病毒性疾病的疫苗。冷等离体等离子体（CAP）是一种依赖活性氧物质来实现理想细胞效果的物理方法，已被证明可有效增强病毒的繁殖能力，其中牛鼻气管炎病毒和马迪尼-达比牛肾细胞分别用作模型病毒和细胞系。CAP 与病毒感染协同作用，将宿主细胞阻滞在 G2/M 期，降低细胞膜电位，增加细胞内钙离子水平，并诱导选择性自噬。此外，CAP 还被证明可抑制病毒触发的免疫信号，如 IRF7 表达。我们提供了有关 CAP 触发的宿主资源最大化以促进病毒繁殖的证据，这对病毒疫苗生产是有利的，并为 CAP 在医疗保健领域的应用开辟了新途径。
