G. Eliava Institute of Bacteriophages, Microbiology and Virology, Tbilisi, GA, USA.
Ilia State University, Tbilisi, GA, USA.
J Appl Microbiol. 2021 Sep;131(3):1344-1359. doi: 10.1111/jam.15030. Epub 2021 Mar 3.
Sulphate-reducing bacteria (SRB) are ecologically important group of anaerobic micro-organisms that can reduce sulphate to form hydrogen sulphide-a toxic gas causing iron corrosion on metal surfaces. In this work, SRB strains were isolated from aquatic environments in the country of Georgia to determine their lysogenicity and the role of temperate phages in host metabolism.
SRB strains were isolated in samples from the Black Sea coast of Georgia. Based on their genetic, cytological and physiological properties of bacteria, 10 Georgian isolates were assigned to the genus Desulfovibrio. Temperate bacteriophages were induced from three out of ten strains by UV-exposure. Comparison of metal (Fe and Cr) reduction and utilization of various carbon sources by the wild-type (lysogenic) bacterial strains and their UV-irradiated counterparts was done.
Temperate phage in the cells of SRB could alter significant functions of bacteria and may have a contribution in the acquisition of different traits by SRB.
This article pointed to a significant role for temperate bacteriophages in the metabolism and metabolic potential of host strains of SRB, which were first isolated from the aquatic environment of Georgia.
硫酸盐还原菌（SRB）是一种生态上重要的厌氧微生物群，能够将硫酸盐还原为有毒的硫化氢气体，导致金属表面的铁腐蚀。在这项工作中，从格鲁吉亚的水生环境中分离出了硫酸盐还原菌菌株，以确定它们的溶原性以及温和噬菌体在宿主代谢中的作用。
从格鲁吉亚黑海沿岸的样本中分离出了硫酸盐还原菌菌株。根据细菌的遗传、细胞学和生理学特性，将 10 个格鲁吉亚分离株分配到脱硫弧菌属。通过紫外线照射从三个菌株中诱导出了温和噬菌体。比较了野生型（溶原性）细菌菌株及其紫外线照射对应物在金属（Fe 和 Cr）还原和利用各种碳源方面的差异。
SRB 细胞中的温和噬菌体可以改变细菌的重要功能，并且可能对 SRB 获得不同特性有贡献。
本文指出，温和噬菌体在 SRB 宿主菌株的代谢和代谢潜力中发挥了重要作用，这些菌株最初是从格鲁吉亚的水生环境中分离出来的。
