Physical Sciences Division, Physical and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.
Nano Lett. 2021 Feb 24;21(4):1636-1642. doi: 10.1021/acs.nanolett.0c04285. Epub 2021 Feb 8.
The fabrication of ordered architectures that intimately integrate polymer, protein, and inorganic components remains difficult. Two promising building blocks to tackle this challenge are peptoids, peptide mimics capable of self-assembly into well-defined structures, and solid-binding peptides, which offer a biological path to controlled inorganic assembly. Here, we report on the synthesis of 3.3-nm-thick thiol-reactive peptoid nanosheets from equimolar mixtures of unmodified and maleimide-derivatized versions of the NbpeNce oligomer, optimize the location of engineered cysteine residues in silica-binding derivatives of superfolder green fluorescent protein for maleimide conjugation, and react the two components to form protein-peptoid hybrids exhibiting partial or uniform protein coverage on both of their sides. Using 10 nm silica nanoparticles, we trigger the stacking of these 2D structures into a multilayered material composed of alternating peptoid, protein, and organic layers. This simple and modular approach to hierarchical hybrid synthesis should prove useful in bioimaging and photocatalysis applications.
制备将聚合物、蛋白质和无机成分紧密集成的有序结构仍然具有挑战性。两种有前途的构建块可以解决此挑战：肽模拟物，能够自组装成具有明确定义结构的肽模拟物，以及固相结合肽，为控制无机组装提供了一种生物学途径。在这里，我们报告了从等摩尔比的未修饰和马来酰亚胺衍生的 NbpeNce 寡聚物混合物合成 3.3nm 厚的巯基反应性肽模拟纳米片，优化了在超折叠绿色荧光蛋白的硅结合衍生物中设计的半胱氨酸残基的位置，以便与马来酰亚胺进行反应，形成在其两侧部分或均匀覆盖蛋白质的蛋白-肽模拟物杂种。使用 10nm 二氧化硅纳米颗粒，我们触发这些 2D 结构堆叠成由交替的肽模拟物、蛋白质和有机层组成的多层材料。这种简单的模块化分层杂化合成方法应该在生物成像和光催化应用中证明是有用的。
