Research Division of Food Functionality, Korea Food Research Institute, Jeollabuk-do 55365, Republic of Korea; Department of Food Science and Technology, Jeonbuk National University, Jeollabuk-do 54896, Republic of Korea.
Department of Food Science and Biotechnology, Kyonggi University, Gyeonggi 16227, Republic of Korea.
Int J Biol Macromol. 2021 Apr 1;175:443-450. doi: 10.1016/j.ijbiomac.2021.02.019. Epub 2021 Feb 5.
Plant-derived polysaccharides possess potential health benefits that improve intestinal health and the immune system. Molokhia leaves have a large amount of mucilage polysaccharide; in the present study, crude polysaccharide extract was prepared from molokhia leaves. The molecular weight of molokhia leaf polysaccharide fraction (MPF) was estimated to be 51.2 × 10 Da. Polysaccharide was methylated and the structure of MPF was mainly composed of rhamnogalacturonan-I structure with side chains, such as galactans and linear glucan (starch), as shown by GC-MS analysis. To study the biofunctional effects of MPF, its prebiotic and intestinal immune-enhancing activities were assayed in vitro. MPF exhibited good prebiotic activity, as shown by its high prebiotic scores, and increased contents of total short-chain fatty acids on five probiotic strains. In addition, MPF showed immune-enhancing activity on Peyer's patches, as revealed by the high bone marrow cell proliferating activity and production of immunoglobulin A and cytokines. These results demonstrate that MPF may be a potential beneficial prebiotic and intestinal immune-enhancer, which may have wide implications in the food industry.
植物来源的多糖具有改善肠道健康和免疫系统的潜在健康益处。蕹菜叶含有大量的粘多糖；在本研究中，从蕹菜叶中制备了粗多糖提取物。蕹菜叶多糖级分（MPF）的分子量估计为 51.2×10 Da。多糖经过甲基化，GC-MS 分析表明 MPF 的结构主要由鼠李半乳糖醛酸聚糖-I 结构和侧链组成，如半乳糖和线性葡聚糖（淀粉）。为了研究 MPF 的生物功能作用，在体外检测了其益生元和肠道免疫增强活性。MPF 表现出良好的益生元活性，其益生元评分高，并增加了五种益生菌菌株的总短链脂肪酸含量。此外，MPF 对派尔集合淋巴结具有免疫增强活性，表现为骨髓细胞增殖活性和免疫球蛋白 A 和细胞因子的产生增加。这些结果表明，MPF 可能是一种有潜力的有益生元和肠道免疫增强剂，这可能在食品工业中有广泛的应用前景。
