Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China; Tianjin Engineering Technology Center of Green Manufacturing Biobased Materials, Tianjin 300071, China.
Bioresour Technol. 2021 May;327:124787. doi: 10.1016/j.biortech.2021.124787. Epub 2021 Feb 1.
Microbial consortia offer an attractive biodegradation strategy for removing hydrocarbons from oil-contaminated sites. In this study, we explored the degradation properties of Acinetobacter venetianus strain RAG-1 (RAG-1). RAG-1 effectively degrades three crude oils with excellent emulsification activity and cell surface hydrophobicity, while exhibiting broad environmental tolerance. RAG-1 accepts a range of alkane substrates (C-C) using three alkane hydroxylases (AlkMa, AlkMb, and AlmA). Bacterial mutant with alkMa or alkMb deletion enhanced degradation of C-C or C-C n-alkanes, respectively. Based on the substrate metabolism of the mutants, adjustable and targeted consortia consisting of ΔalkMa/almA and ΔalkMb were constructed, achieving enhanced degradation (10 days) of light crude oil (73.42% to 88.65%), viscous crude oil (68.40% to 90.05%), and high waxy crude oil (47.46% to 60.52%) compared with the single wild-type strain. The degradation properties of RAG-1 and the engineered consortia strategy may have potential use in microbial biodegradation applications.
微生物群落为去除受石油污染场地的碳氢化合物提供了一种有吸引力的生物降解策略。在这项研究中，我们探索了不动杆菌菌株 RAG-1（RAG-1）的降解特性。RAG-1 有效地降解了三种原油，具有出色的乳化活性和细胞表面疏水性，同时表现出广泛的环境耐受性。RAG-1 使用三种烷烃羟化酶（AlkMa、AlkMb 和 AlmA）接受一系列烷烃底物（C-C）。具有 alkMa 或 alkMb 缺失的细菌突变体分别增强了 C-C 或 C-Cn-烷烃的降解。基于突变体的底物代谢，构建了由 ΔalkMa/almA 和 ΔalkMb 组成的可调且靶向的群落，与单一野生型菌株相比，实现了轻质原油（73.42%至 88.65%）、粘性原油（68.40%至 90.05%）和高含蜡原油（47.46%至 60.52%）的增强降解（10 天）。RAG-1 的降解特性和工程化群落策略可能在微生物生物降解应用中具有潜在用途。
