Liu Renna, Xu Fanli, Wang Lu, Liu Mengxue, Cao Xueyan, Shi Xiangyang, Guo Rui
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China.
Nanomaterials (Basel). 2021 Feb 4;11(2):394. doi: 10.3390/nano11020394.
Theranostic nanoplatforms combining photosensitizers and anticancer drugs have aroused wide interest due to the real-time photoacoustic (PA) imaging capability and improved therapeutic efficacy by the synergistic effect of chemotherapy and phototherapy. In this study, polydopamine (PDA) coated laponite (LAP) nanoplatforms were synthesized to efficiently load indocyanine green (ICG) and doxorubicin (DOX), and modified with polyethylene glycol-arginine-glycine-aspartic acid (PEG-RGD) for PA imaging-guided chemo-phototherapy of cancer cells overexpressing αβ integrin. The formed ICG/LAP-PDA-PEG-RGD/DOX nanoplatforms showed significantly higher photothermal conversion efficiency than ICG solution and excellent PA imaging capability, and could release DOX in a pH-sensitive and NIR laser-triggered way, which is highly desirable feature in precision chemotherapy. In addition, the ICG/LAP-PDA-PEG-RGD/DOX nanoplatforms could be uptake by cancer cells overexpressing αβ integrin with high specificity, and thus serve as a targeted contrast agent for in vivo PA imaging of cancer. In vivo experiments with 4T1 tumor-bearing mouse model demonstrated that ICG/LAP-PDA-PEG-RGD/DOX nanoplatforms exhibited much stronger therapeutic effect and higher survival rate than monotherapy due to the synergetic chemo-phototherapy under NIR laser irradiation. Therefore, the reported ICG/LAP-PDA-PEG-RGD/DOX represents a promising theranostic nanoplatform for high effectiveness PA imaging-guided chemo-phototherapy of cancer cells overexpressing αβ integrin.
结合光敏剂和抗癌药物的诊疗纳米平台因其具有实时光声（PA）成像能力以及化疗和光疗协同效应所带来的更高治疗效果而引起了广泛关注。在本研究中，合成了聚多巴胺（PDA）包覆的锂皂石（LAP）纳米平台，以有效负载吲哚菁绿（ICG）和阿霉素（DOX），并用聚乙二醇-精氨酸-甘氨酸-天冬氨酸（PEG-RGD）进行修饰，用于对过表达αβ整合素的癌细胞进行PA成像引导的化疗-光疗。所形成的ICG/LAP-PDA-PEG-RGD/DOX纳米平台显示出比ICG溶液显著更高的光热转换效率和出色的PA成像能力，并且能够以pH敏感和近红外激光触发的方式释放DOX，这是精准化疗中非常理想的特性。此外，ICG/LAP-PDA-PEG-RGD/DOX纳米平台能够被过表达αβ整合素的癌细胞高特异性摄取，因此可作为癌症体内PA成像的靶向造影剂。在4T1荷瘤小鼠模型上进行的体内实验表明，由于在近红外激光照射下的协同化疗-光疗，ICG/LAP-PDA-PEG-RGD/DOX纳米平台比单一疗法表现出更强的治疗效果和更高的存活率。因此，所报道的ICG/LAP-PDA-PEG-RGD/DOX代表了一种有前景的诊疗纳米平台，用于对过表达αβ整合素的癌细胞进行高效PA成像引导的化疗-光疗。
