Ge Tongling, Ekbataniamiri Fatemeh, Johnson Steven B, Larkin Robert P, Hao Jianjun
School of Food and Agriculture, University of Maine, Orono, ME 04469, USA.
Cooperative Extension, University of Maine, Orono, ME 04469, USA.
Microorganisms. 2021 Feb 4;9(2):316. doi: 10.3390/microorganisms9020316.
and spp. both cause blackleg and soft rot of potato, which can be a yield-reducing factor to potato production. The purpose of this study was to examine the interaction between these two bacterial genera causing potato infection, and subsequent disease development and yield responses under field conditions. Analysis of 883 potato samples collected in Northeastern USA using polymerase chain reaction determined that and were found in 38.1% and 53.3% of all samples, respectively, and that 20.6% of samples contained both and . To further investigate the relationship between the two bacterial species and their interaction, field trials were established. Potato seed pieces of "Russet Burbank", "Lamoka", and "Atlantic" were inoculated with bacterial suspension of at 10 colony-forming unite (CFU)/mL using a vacuum infiltration method, air dried, and then planted in the field. Two-year results showed that there was a high correlation ( < 0.01) between yield loss and percent of inoculated seed pieces. In a secondary field trial conducted in 2018 and 2019, seed pieces of potato "Shepody", "Lamoka" and "Atlantic" were inoculated with , , or mixture of both species, and then planted. In 2019, disease severity index, as measured by the most sensitive variety "Lamoka", was 16.2 with inoculation, 10.4 with , 25.4 with inoculation with both bacteria. Two-year data had a similar trend. Thus, was more virulent than . , but the co-inoculation of the two species resulted in increased disease severity compared to single-species inoculation with either pathogen.
[细菌名称1]和[细菌名称2]都会引发马铃薯黑胫病和软腐病，这可能是马铃薯生产中的一个减产因素。本研究的目的是考察这两种导致马铃薯感染的细菌属之间的相互作用，以及在田间条件下随后的病害发展和产量反应。利用聚合酶链反应对在美国东北部采集的883份马铃薯样本进行分析，结果确定，在所有样本中，[细菌名称1]和[细菌名称2]的检出率分别为38.1%和53.3%，20.6%的样本同时含有[细菌名称1]和[细菌名称2]。为了进一步研究这两种细菌之间的关系及其相互作用，开展了田间试验。采用真空渗透法，用浓度为10个菌落形成单位（CFU）/毫升的[细菌名称1]细菌悬液接种“褐皮伯班克”“拉莫卡”和“大西洋”品种的马铃薯种薯块，风干后种植于田间。两年的结果表明，产量损失与接种种薯块的比例之间存在高度相关性（P<0.01）。在2018年和2019年进行的一项二级田间试验中，用[细菌名称1]、[细菌名称2]或两种细菌的混合物接种“谢泼迪”“拉莫卡”和“大西洋”品种的马铃薯种薯块，然后种植。2019年，以最敏感品种“拉莫卡”衡量的病情严重指数，接种[细菌名称1]时为16.2，接种[细菌名称2]时为10.4，两种细菌共同接种时为25.4。两年的数据呈现类似趋势。因此，[细菌名称1]的毒性比[细菌名称2]更强。但是，与单一病原体接种相比，两种细菌共同接种导致病情严重程度增加。
