Amir Muhammad, Hussain Abrar, Asif Muhammad, Ahmed Sagheer, Alam Hina, Moga Marius Alexandru, Cocuz Maria Elena, Marceanu Luigi, Blidaru Alexandru
Department of Biotechnology, BUITEMS, Baluchistan University of Information & Technology, Engineering and Management Sciences (BUITEMS), Quetta 87300, Pakistan.
Office of Research Innovation and Commercialization, Baluchistan University of Information & Technology, Engineering and Management Sciences (BUITEMS), Quetta 87300, Pakistan.
Microorganisms. 2021 Feb 4;9(2):323. doi: 10.3390/microorganisms9020323.
Dengue fever is among the most common vector-borne diseases. Dengue virus (DENV), responsible for dengue fever as well as dengue hemorrhagic fever, belongs to the genus flavivirus and family Flaviviridae. Flaviviruses infect various vertebrate species and arthropods and are also responsible for diseases in birds, wild animals, and primates. DENV consists of a single-stranded, positive-sense RNA genome ~11 kb in size. Complete genome and partial gene sequences of geographically distinct DENV-3 strains were retrieved from the GenBank database. The evolutionary divergence of the 33 whole-genome and individual gene sequences of the nucleotides and amino acids of DENV-3 strains were generated with the maximum likelihood (ML) and Bayesian phylogenetic study (BEAST) methods using the MEGA 7 software. The genome size varied from 10,484 to 10,724 nucleotides among the strains with distinct geographical backgrounds belonging to Central America, South-Central Asia, and Eastern Asia. A phylogenetic analysis of the nucleotide and amino acid sequences of these DENV-3 isolates revealed extensive differences in the topologies due to PrM/M, NS1, NS2B, and NS3 genes. These results suggest substantial variation in the evolutionary pathways of the studied genes and genomes.
登革热是最常见的媒介传播疾病之一。导致登革热以及登革出血热的登革病毒（DENV）属于黄病毒属和黄病毒科。黄病毒感染各种脊椎动物物种和节肢动物，也会引发鸟类、野生动物和灵长类动物的疾病。DENV由一个大小约为11 kb的单链正义RNA基因组组成。从GenBank数据库中检索了地理上不同的DENV - 3毒株的完整基因组和部分基因序列。使用MEGA 7软件，通过最大似然法（ML）和贝叶斯系统发育研究（BEAST）方法，对DENV - 3毒株的33个全基因组以及核苷酸和氨基酸的单个基因序列进行了进化分歧分析。在属于中美洲、中南亚和东亚的具有不同地理背景的毒株中，基因组大小从10,484到10,724个核苷酸不等。对这些DENV - 3分离株的核苷酸和氨基酸序列进行的系统发育分析显示，由于包膜蛋白前体/膜蛋白（PrM/M）、非结构蛋白1（NS1）、非结构蛋白2B（NS2B）和非结构蛋白3（NS3）基因，拓扑结构存在广泛差异。这些结果表明，所研究的基因和基因组的进化途径存在显著差异。
