Miranda Jorge, Mattar Salim
Instituto de Investigaciones Biológicas del Trópico, Facultad de Medicina Veterinaria y Zootecnia, Universidad de Córdoba, Montería, Colombia.
Trop Biomed. 2015 Dec 1;32(4):726-735.
Anaplasma and Ehrlichia species are important tick-borne pathogens that cause disease in cattle, dogs, horses and humans; with little information available about these agents in Colombia. The aim of this study was to provide molecular evidence for the presence of Anaplasma sp. and Ehrlichia sp. species in ticks collected from horses, dogs and cattle in Cordoba, Colombia. In this study, 1.105 ticks were removed from 226 zebu cattle (Bos indicus), 87 dogs (Canis lupus familiaris) and 19 horses (Equus caballus), from different localities of Cordoba. Ticks were identified taxonomically and PCR assays were used for the amplification of Anaplasma sp. and Ehrlichia sp. DNA. The amplification products were sequenced and analyzed. From the 1,105 ticks examined, 679 (61.5%) were Rhipicephalus microplus, collected from cattle, 353 (32%) were Rhipicephalus sanguineus from dogs and 73 (6,6%) Dermacentor nitens collected on horses; 332 pools were formed to develop the PCR assay. Anaplasma sp. and Ehrlichia sp. DNA was detected in 5.7% (19/332 pools). Direct sequencing of amplicons showed that seven sequences had similarities between 99-100% with Anaplasma marginale, one sequence showed 100% identical with Anaplasma phagocytophilum, seven sequences showed 100% identity with Ehrlichia ewingii, one sequence had 100% identity with E. chaffensis and three sequences showed similarities of 99% and 95% with Ehrlichia mineirensis and Ehrlichia canis respectively. In conclusion, several tick-borne pathogens identified in this survey suggests that there is a risk for the emergence of tick-borne diseases in domestic animals and humans in Colombia. Our data provides evidence of Ehrlichia and Anaplasma species circulating in ticks from Cordoba.
无形体属和埃立克体属物种是重要的蜱传病原体，可在牛、狗、马和人类中引发疾病；而关于哥伦比亚这些病原体的信息却很少。本研究的目的是为哥伦比亚科尔多瓦地区从马、狗和牛身上采集的蜱中存在无形体属和埃立克体属物种提供分子证据。在本研究中，从科尔多瓦不同地区的226头瘤牛（印度野牛）、87只狗（家犬）和19匹马（马）身上采集了1105只蜱。对蜱进行分类鉴定，并使用聚合酶链反应（PCR）检测法扩增无形体属和埃立克体属的DNA。对扩增产物进行测序和分析。在所检查的1105只蜱中，679只（61.5%）是微小扇头蜱，采自牛；353只（32%）是血红扇头蜱，采自狗；73只（6.6%）是美洲钝缘蜱，采自马；共形成332个样本池用于PCR检测。在5.7%（19/332个样本池）中检测到无形体属和埃立克体属的DNA。扩增子的直接测序显示，7个序列与边缘无形体的相似度在99%至100%之间，1个序列与嗜吞噬细胞无形体100%相同，7个序列与尤因埃立克体100%相同，1个序列与查菲埃立克体100%相同，3个序列分别与米内罗埃立克体和犬埃立克体的相似度为99%和95%。总之，本次调查中鉴定出的几种蜱传病原体表明，哥伦比亚的家畜和人类存在蜱传疾病出现的风险。我们的数据为埃立克体属和无形体属物种在科尔多瓦蜱中传播提供了证据。
