Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, P.O. Box. 7144169155, Shiraz, Fars, Iran.
Department of Basic Sciences, School of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran.
BMC Complement Med Ther. 2021 Feb 8;21(1):55. doi: 10.1186/s12906-021-03229-x.
Polycystic ovary syndrome (PCOS) is one of the most prevalent endocrinopathies in women during the reproductive age. Herbal medicines are used increasingly alone or in supplement with chemical medicines for the treatment of different diseases and dysfunctions. This study was aimed to evaluate the effects of lutein and nettle (Urtica dioica) extract on the biochemical parameters and the reproductive function in the PCOS model of mice.
Following the induction of PCOS by dehydroepiandrosterone (DHEA), the mice (n = 98) were randomly assigned into seven groups, each consisting of fourteen mice; the groups were included control group (received solvent), PCOS group (received 6 mg/100 g B.W/day IP, DHEA for 21 days), PCOS+ Nettle extract (200 and 400 mg/kg), PCOS+ Lutein (125 and 250 mg/kg), and PCOS+ NL (200 mg/kg nettle extract and 125 mg/kg lutein). The nettle extract and lutein were administrated using gavage for 30 consecutive days after PCOS induction. Malondialdehyde (MDA), total antioxidant capacity (TAC), and estrogen were measured in serum, ovary, and uterus samples by the ELISA method. The total number of oocytes, oocyte quality, fertilization rate, 2-cell blastocyst, and arrested embryos (type I, type II, and type III) were also investigated.
A combination treatment of the nettle and lutein produced the lowest concentration of MDA in comparison to other groups which affected by the PCOS. The lowest level of TAC was observed in the PCOS group without treatment. The number of oocytes, oocyte quality, fertilization rate, and 2-cell blastocyst were significantly higher in the control group, but the lowest values were observed in the PCOS group without any treatment.
The most favorable findings include improving antioxidant capacity, oocyte and embryo quality were observed in the PCOS+ 125 L group.
多囊卵巢综合征（PCOS）是育龄妇女中最常见的内分泌疾病之一。草药被越来越多地单独使用或与化学药物联合用于治疗各种疾病和功能障碍。本研究旨在评估叶黄素和荨麻（荨麻）提取物对多囊卵巢综合征模型小鼠生化参数和生殖功能的影响。
用脱氢表雄酮（DHEA）诱导 PCOS 后，将 98 只小鼠随机分为 7 组，每组 14 只；对照组（给予溶剂）、PCOS 组（给予 6mg/100g B.W/天 IP，DHEA 21 天）、PCOS+荨麻提取物（200 和 400mg/kg）、PCOS+叶黄素（125 和 250mg/kg）和 PCOS+NL（200mg/kg 荨麻提取物和 125mg/kg 叶黄素）。在诱导 PCOS 后，连续 30 天通过灌胃给予荨麻提取物和叶黄素。采用 ELISA 法测定血清、卵巢和子宫样本中丙二醛（MDA）、总抗氧化能力（TAC）和雌激素的含量。还研究了卵母细胞总数、卵母细胞质量、受精率、2-细胞胚泡和阻滞胚胎（I 型、II 型和 III 型）的数量。
与其他受 PCOS 影响的组相比，荨麻和叶黄素联合治疗组 MDA 浓度最低。未治疗的 PCOS 组 TAC 水平最低。对照组的卵母细胞数量、卵母细胞质量、受精率和 2-细胞胚泡数均显著升高，但未治疗的 PCOS 组最低。
最有利的发现包括提高抗氧化能力，改善卵母细胞和胚胎质量，在 PCOS+125L 组观察到。
